HepG2 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 300198

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	HepG2 cells, a hepatoblastoma cell line, are a cornerstone in biological science, particularly in liver cancer research. The HepG2 cell line was first isolated in 1975 and initially misclassified as hepatocellular carcinoma, with the HepG2 cell line origin as hepatoblastoma being recognized later, clarifying years of scientific ambiguity. Human hepatic cell lines such as HepG2 are commonly used as in vitro models for primary human hepatocytes. These cell lines offer advantages such as indefinite proliferation, stable phenotype, easy accessibility, and ease of manipulation. However, they exhibit reduced expression of some metabolic functions compared to primary hepatocytes. Derived from hepatocellular carcinoma, HepG2 cells proliferate quickly and have an epithelial-like morphology, performing many specialized hepatic functions. Despite these differences, HepG2 cells are widely used in studying drug metabolism and toxicity, thanks to their resemblance to hepatocellular carcinoma and hepatoblastoma cells in terms of drug metabolism and transport proteins. HepG2 is a human liver cancer cell line often used in research, including studies on drug metabolism and toxicity. However, one of the limitations of hepatoma HepG2 cells is their altered expression of certain liver-specific functions, including the expression of cytochrome P450 enzymes. Cytochrome P450 enzymes are essential for the metabolism of xenobiotics (foreign compounds such as drugs and carcinogens) in the liver. The altered or reduced expression of these enzymes in HepG2 cells can affect their ability to accurately model the metabolism and elimination of xenobiotics, which is a critical aspect of liver function. The HepG2 cell line, alongside other hepatoma cell lines such as the Hep3B and human hepatoma HepaRG cell lines, contributes to a broader understanding of human liver carcinoma cells. The cell line stands out for its versatility, serving as an optimal choice for stable cell line generation, transfection studies, drug metabolism, and hepatotoxicity studies. Furthermore, the HepG2 cell line is pivotal in a range of applications, from 3D cell culture to high-throughput screening and toxicology.
Organismo	Human
Tecido	Liver
Doença	Hepatocellular carcinoma
Aplicações	This cell line is an optimal choice for transfection. Further, the HepG2 cells offer an array of applications, ranging from 3D cell culture and cancer research to high-throughput screening and toxicology.
Sinônimos	HEP-G2, Hep G2, HEP G2, Hep-G2, HEPG2

Idade	15 years
Gênero	Male
Etnia	Caucasian
Morfologia	Epithelial-like
Propriedades de crescimento	Adherent

Referência	HepG2 (Cytion catalog number 300198)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_0027

Receptores expressos	Insulin, insulin-like growth factor II (IGF II)
Expressão de proteínas	P53 positive
Tumorigênico	No
Produtos	Albumin, alpha-fetoprotein (alpha fetoprotein), alpha1 acid glycoprotein (alpha-1 acid glycoprotein), alpha1 antitrypsin (alpha-1-antitrypsin), alpha1 antichymotrypsin, (alpha-1-antichymotrypsin), alpha2 HS glycoprotein (alpha-2-HS- glycoprotein), alpha2 macroglobulin (alpha-2-macroglobulin), beta lipoprotein (beta-lipoprotein), ceruloplasmin, C4 and C3 activator, fibrinogen, haptoglobin, plasminogen, retinol binding protein (retinolbinding protein), transferrin
Cariótipo	Modal number = 55 (range = 50 to 60), has a rearranged chromosome 1

Meio de cultura	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Tempo de duplicação	48 hours
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densidade de semeadura	2 to 3 x 10⁴ cells/cm² during routine culture
Renovação de fluidos	2 to 3 times per week
Recuperação pós-degelo	Start culture using the complete contents of the cryovial in 2xT25 cell culture flasks. The cells will recover within 48 to 72 hours.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300198-240624	Certificado de Análise	23. May. 2025	300198
300198-260523	Certificado de Análise	23. May. 2025	300198
300198-300525	Certificado de Análise	21. Jul. 2025	300198

HepG2 Cells

Essential facts on HepG2 cells

Characteristics of the HepG2 cell line

Dados regulatórios

Genomics of HepG2 human hepatoma cells

Handling of Hep G2 cells

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)