SN12C Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 800,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305629

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The SN12C cell line is a human renal cell carcinoma (RCC) model derived from a primary tumor of a 43-year-old male patient. This cell line has been widely used in cancer research, particularly for investigating the biology and therapeutic targeting of RCC. SN12C cells are adherent in culture and exhibit properties consistent with epithelial morphology. The cell line is also part of the NCI-60 panel, making it extensively characterized in terms of its genomic, transcriptomic, and proteomic profiles. SN12C cells have been employed in studies exploring tumor progression and metastasis. When implanted orthotopically in the renal subcapsule of nude mice, SN12C cells form primary tumors and have been shown to produce lung metastases. These metastases have been used to derive variant cell lines with enhanced metastatic potential, making SN12C a valuable model for studying the genetic and phenotypic factors driving metastasis. The cell line has also been analyzed for mutations in key oncogenes and tumor suppressors, revealing its distinct genetic alterations, including potential oncogenic drivers of RCC. SN12C has been utilized to evaluate responses to chemotherapy and targeted therapies, contributing to the understanding of RCC's drug resistance mechanisms. Its inclusion in the NCI-60 panel has enabled high-throughput drug screening and molecular profiling, aiding the identification of compounds with selective activity against RCC. These attributes make SN12C an indispensable tool for advancing both basic and translational RCC research.
Organismo	Human
Tecido	Kidney
Doença	Renal cell carcinoma
Sinônimos	SN-12C, SN12 C

Idade	Unspecified
Gênero	Male
Etnia	Caucasian
Morfologia	Epithelial-like
Tipo de célula	Renal cell
Propriedades de crescimento	Adherent, monolayer

Referência	SN12C (Cytion catalog number 305629)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_1705

Perfil mutacional	Mutation: TP53, Simple, p.Glu336Ter (c.1006G>T), Homozygous

Meio de cultura	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suplementos	Supplement the medium with 10% FBS
Tempo de duplicação	26-30 hours
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305629-170325	Certificado de Análise	23. May. 2025	305629
305629-300126	Certificado de Análise	05. Mar. 2026	305629

SN12C Cells

Informações gerais

Características

Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)