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22RV1 Cells

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305037

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	The 22Rv1 cell line is a human prostate carcinoma cell line that was established from a xenograft initiated by the inoculation of a hormone-refractory prostate cancer cell line, CWR22, into athymic nude mice. The CWR22 xenograft was derived from a primary prostate carcinoma. Upon regression after castration and subsequent relapse, the 22Rv1 cell line was established from the relapsed tumor, which exhibited androgen-independent growth. 22Rv1 cells express the androgen receptor (AR) and prostate-specific antigen (PSA), essential markers in prostate cancer research and therapeutic targeting. Notably, this cell line contains a variant form of the AR known as AR-V7. This splice variant lacks the ligand-binding domain, enabling it to remain constitutively active and contribute to the androgen-independent proliferation of 22Rv1 cells, a critical aspect of castration-resistant prostate cancer (CRPC). The 22Rv1 cell line is extensively used to investigate the mechanisms underlying the transition from androgen-dependent to androgen-independent prostate cancer growth, a key challenge in the treatment of advanced prostate cancer. 22Rv1 cells have facilitated significant advancements in understanding the molecular biology of CRPC, including the role of AR variants in resistance to androgen deprivation therapy (ADT) and the development of novel therapeutic strategies aimed at overcoming this resistance. In summary, the 22Rv1 cell line, serves as a critical model for studying CRPC. Exhibiting androgen-independent growth, these cells express key prostate cancer markers such as AR and PSA, and notably contain the AR-V7 variant, which is constitutively active due to the absence of the ligand-binding domain. The 22Rv1 cell line's unique properties make it invaluable for exploring the transition from androgen-dependent to independent growth in prostate cancer, and thereby aid in the development of new therapeutic approaches to tackle advanced stages of the disease.
Organisme	Human
Tissu	Prostate
Maladie	Prostate carcinoma
Synonymes	22Rv1, 22Rv-1, 22rV1, CWR-22rv1, CWR22-Rv1, CWR22R-V1, CWR22-R1, CWR22Rv1, CWR22R

Âge	Adult
Genre	Male
Origine ethnique	European
Morphologie	Epithelial
Propriétés de croissance	Adherent

Référence	22RV1 (Cytion catalog number 305037)
Niveau de biosécurité	2
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_1045

Expression de l'antigène	Prostate-specific antigen (PSA)
Tumorigène	Yes

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Temps de doublement	40 to 60 hours
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305037-021222	Certificat d'analyse	23. May. 2025	305037

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