SW480 Cells

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 300302

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Certificat d'analyse (CoA)

Description	The SW480 cell line originated from a surgical specimen of a primary tumor of a moderately differentiated colon adenocarcinoma.
Organisme	Human
Tissu	Colon
Maladie	Adenocarcinoma, Grade IV, Dukes' type B.
Synonymes	SW480, SW 480, SW480E

Âge	50 years
Genre	Male
Origine ethnique	Caucasian
Morphologie	Epithelial-like
Propriétés de croissance	Adherent

Référence	SW-480 (Cytion catalog number 300302)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_0546

Récepteurs exprimés	Epidermal growth factor (EGF), keratin (immunoperoxidase staining). Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed.
Expression des protéines	The cells express elevated levels of p53 protein.
Expression de l'antigène	HLA A2, B8, B17, blood type A, Rh+. The line is negative for CSAp (CSAp-) and colon antigen 3
Isoenzymes	G6PD, B, PGM1, 2, PGM3, 1, 6PGD, A, PEP-D, 1, ES-D, 1
Tumorigène	Yes, in nude mice
Virus	Reverse transcriptase negative
Sensibilité aux virus	Human immunodeficiency virus (HIV, LAV)
Produits	Carcinoembryonic antigen (CEA) 0.7 ng/106 cells/10 days, keratin, TGF-β. The cells have been reported to produce GM-CSF.
Profil mutationnel	SW-480 cells carry a homozygous Kras mutation in codon 12: GGT(Wt Gly) >GTT(Val). There is a G->A mutation in codon 273 of the p53 gene resulting in an Arg->His substitution and a C->T mutation in codon 309 resulting in a Pro->Ser substitution.

Milieu de culture	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Temps de doublement	20 to 25 hours
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densité de semis	1 x 10⁴ cells/cm²
Renouvellement des fluides	1 to 2 times per week
Rétablissement après le dégel	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
300302-080724	Certificat d'analyse	23. May. 2025	300302

SW480 Cells

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Certificat d'analyse (CoA)

Organisme	Human
Tissu	Urinary bladder
Maladie	Bladder carcinoma