Panc02-Luc Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

1 104,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305706

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	Panc02-Luc is a luciferase-expressing derivative of the Panc02 murine pancreatic adenocarcinoma cell line. Panc02 cells originate from chemically induced pancreatic ductal adenocarcinoma in mice and are widely used as a syngeneic model of pancreatic cancer in immunocompetent murine hosts. The introduction of a luciferase reporter enables highly sensitive bioluminescent imaging of tumor cells in vitro and in vivo, facilitating noninvasive longitudinal monitoring of tumor growth, metastatic dissemination, and therapeutic response. These properties make Panc02-Luc a valuable platform for pancreatic cancer biology, immuno-oncology, and preclinical drug development studies. Panc02-Luc cells are commonly utilized in orthotopic and subcutaneous mouse tumor models to investigate tumor progression, stromal interactions, immune cell infiltration, and mechanisms of resistance to chemotherapy or immunotherapy. Because Panc02 tumors can be established in syngeneic mouse strains with an intact immune system, the model is particularly useful for evaluating checkpoint inhibitors, adoptive cell therapies, cancer vaccines, and combination treatment strategies. Luciferase-based imaging enables repeated quantitative assessment of tumor burden in living animals, reducing experimental variability and supporting real-time evaluation of treatment efficacy. Panc02-Luc cells are used for studies of pancreatic tumor cell proliferation, migration, invasion, cytokine signaling, metabolic adaptation, and apoptosis. The biological behavior of the model may vary depending on the luciferase construct, promoter system, and clonal selection strategy used during engineering. Further characterization data, including reporter stability, luminescence intensity, and metastatic potential, may be important for specialized experimental applications.
Organisme	Mouse
Tissu	Pancreas
Maladie	Mouse pancreatic ductal adenocarcinoma
Synonymes	Luciferase Reporter Panc02 Cell Line

Race/Sous-espèce	C57BL/6
Âge	Unspecified
Genre	Male
Propriétés de croissance	Adherent

Référence	Panc02-Luc (Cytion catalog number 305706)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	10090
Cellosaurus - Numéro d'enregistrement	CVCL_E3IB

Expression des protéines	Luc

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Temps de doublement	24-48 hours
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densité de semis	1 to 3 x 10⁴ cells/cm²
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305706-100426	Certificat d'analyse	15. May. 2026	305706

Panc02-Luc Cells

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Certificat d'analyse (CoA)