HEK293FT Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

1 104,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305275

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	The HEK293FT cell line is a derivative of the HEK293 cell line, originally derived from human embryonic kidney cells. The "FT" designation indicates that these cells have been transfected with the SV40 large T-antigen gene, which enhances their ability to replicate plasmid vectors containing the SV40 origin of replication. This modification makes 293FT cells particularly useful for high-efficiency production of viral vectors, such as lentiviruses and adenoviruses, and for transfection studies in molecular biology and gene therapy research. HEK293FT cells exhibit an epithelial morphology and grow rapidly in culture, providing a robust and reliable system for producing high-titer viral stocks. They retain many of the characteristics of the parental HEK293 cells, including high transfection efficiency and the ability to support the replication of recombinant viruses. Researchers utilize 293FT cells to produce viral vectors for gene delivery, to study gene function and regulation, and to develop gene therapies for various diseases. Their role in the production of viral vectors makes 293FT cells a cornerstone in the fields of gene therapy, functional genomics, and molecular cloning, facilitating the advancement of research and therapeutic development.
Organisme	Human
Tissu	Fetal Kidney
Synonymes	HEK293-FT, HEK-293FT, HEK 293FT, HEK-293-FT, HEK293FT, 293-FT, FT-293

Âge	Fetus
Genre	Female
Morphologie	Epithelial
Propriétés de croissance	Adherent

Référence	HEK293FT (Cytion catalog number 305275)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_6911
Statut OGM	GMO-S1: This HEK293-derived cell line (293-FT) contains an SV40 expression plasmid with neomycin selection, supporting enhanced proliferation and transfection efficiency. The construct provides stable SV40. This classification applies only within Germany and may differ elsewhere.

Expression de l'antigène	SV40 large T antigen, Adenovirus early region 1A (E1A)
Virus	Transformant: Adenovirus 5, Simian virus 40 (SV40)

Milieu de culture	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suppléments	Supplement the medium with 10% FBS.
Réactif de dissociation	Accutase
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densité de semis	2 to 5 x 10⁴ cells/cm²
Renouvellement des fluides	2 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305275-281024	Certificat d'analyse	18. Aug. 2025	305275
305275-301025	Certificat d'analyse	05. Dec. 2025	305275

HEK293FT Cells

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Données biomoléculaires

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Contrôle de la qualité et analyse moléculaire

Certificat d'analyse (CoA)