PY8119-GFP

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

1 104,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305857

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	PY8119-GFP cells are a fluorescently labeled derivative of the murine PY8119 breast cancer cell line, which originates from a spontaneous mammary tumor in a mouse model. The parental PY8119 line is commonly associated with genetically engineered mouse models of breast cancer and is used to study tumor progression, immune interactions, and therapeutic responses in a syngeneic context. Stable expression of green fluorescent protein (GFP) enables direct visualization of tumor cell behavior, facilitating real-time tracking of proliferation, migration, and cellular interactions both in vitro and in vivo. The GFP modification makes PY8119-GFP cells particularly well suited for applications such as live-cell imaging, intravital microscopy, and metastasis studies in immunocompetent mouse models. These cells retain key characteristics of the parental line, including aggressive growth and the ability to form tumors when implanted into syngeneic hosts. As a result, PY8119-GFP cells are widely used to investigate tumor–immune system dynamics, evaluate anti-cancer therapies, and study mechanisms of invasion and dissemination. However, as with all fluorescently modified lines, careful validation is recommended to ensure that GFP expression does not alter baseline cellular behavior.
Organisme	Mouse
Tissu	Mammary gland
Maladie	Malignant neoplasms of the mouse mammary gland
Synonymes	P years8119

Âge	Adult
Genre	Female
Morphologie	Epithelial-like
Type de cellule	Mesenchymal-like cell
Propriétés de croissance	Adherent

Référence	PY8119-GFP (Cytion catalog number 305857)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	10090

Expression des protéines	GFP

Milieu de culture	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suppléments	Supplement the medium with 10% FBS
Densité de semis	1.5-4 X 10⁴ cells/cm2
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305857-040326	Certificat d'analyse	08. Apr. 2026	305857

PY8119-GFP

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Certificat d'analyse (CoA)