Panc02 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 300501

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The Panc02 cell line is a widely used murine model for studying pancreatic ductal adenocarcinoma (PDAC), the most common and aggressive form of pancreatic cancer. Panc02 cells were originally derived from a chemically induced pancreatic tumor in a C57BL/6 mouse. This cell line is highly relevant in preclinical research because it can be implanted orthotopically in syngeneic mice, mimicking the natural tumor environment and offering insights into the immune responses and therapeutic resistance mechanisms of PDAC. Research using Panc02 has provided significant insights into PDAC’s immunosuppressive microenvironment. One study showed that Panc02 tumors are heavily infiltrated by regulatory T cells (Tregs), which suppress the antitumor immune response. Treatment with low-dose gemcitabine was found to selectively deplete Tregs in Panc02 tumor-bearing mice, leading to an enhanced antitumor immune response and a modest increase in survival. This suggests that immunomodulation could be a promising therapeutic strategy for PDAC. In addition to immunotherapy studies, Panc02 has also been used to investigate necroptosis, a form of programmed cell death. Inhibition of Aurora Kinase A in Panc02 cells has been shown to induce necroptosis, which is important for overcoming resistance to apoptosis in PDAC. This provides a potential therapeutic approach to target apoptosis-resistant cancer cells by promoting non-apoptotic cell death pathways.
Organismo	Mouse
Tejido	Pancreas
Enfermedad	Mouse pancreatic ductal adenocarcinoma
Sinónimos	Panc-02, Panc 02, Pan02, PAN 02, Panc02-H0

Raza/Subespecie	C57BL/6
Edad	Unspecified
Género	Male
Propiedades de crecimiento	Adherent

Referencia	Panc02 (Cytion catalog number 300501)
Nivel de bioseguridad	1
NCBI_TaxID	10090
N.º de acceso de Cellosaurus	CVCL_D627

Medio de cultivo	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
300501-060624	Certificado de análisis	23. May. 2025	300502
300501-191224	Certificado de análisis	26. May. 2025	300502
300501-230925	Certificado de análisis	05. Dec. 2025	300501
300501-230226	Certificado de análisis	25. Mar. 2026	300501

Panc02 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)