NCI-H3122 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 300484

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The NCI-H3122 cell line is derived from non-small-cell lung cancer (NSCLC) and is characterized by the presence of the EML4-ALK fusion gene, which results from a chromosomal translocation between echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK). This fusion drives oncogenic signaling and makes NCI-H3122 cells highly dependent on ALK signaling for survival, known as "ALK-addicted." NCI-H3122 has become a key model for studying targeted therapies, particularly for ALK inhibitors like crizotinib. Studies have shown that NCI-H3122 cells are sensitive to crizotinib, which inhibits ALK phosphorylation and its downstream targets such as the AKT and ERK pathways. However, resistance to crizotinib often develops, typically due to alternative signaling pathways like the activation of the epidermal growth factor receptor (EGFR). This resistance mechanism has been confirmed in NCI-H3122 resistant variants, where increased EGFR phosphorylation was observed, and dual inhibition of ALK and EGFR using crizotinib and EGFR inhibitors such as afatinib or erlotinib was shown to overcome the resistance. NCI-H3122 is frequently used to explore combination therapies aimed at preventing or reversing drug resistance. For instance, targeting both ALK and EGFR pathways has been a successful strategy in preclinical models, and this dual inhibition has been suggested as a potential therapeutic approach for ALK-positive, crizotinib-resistant NSCLC patients.
Organismo	Human
Tejido	Lung
Enfermedad	Adenocarcinoma
Sinónimos	NCI-H3122, H-3122, NCIH3122

Género	Male
Origen étnico	Caucasian
Propiedades de crecimiento	Adherent

Referencia	NCI-H3122 (Cytion catalog number 300484)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_5160

Medio de cultivo	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
300484-170724	Certificado de análisis	23. May. 2025	300484
300484-050923	Certificado de análisis	18. Aug. 2025	300484
300484-110625	Certificado de análisis	18. Aug. 2025	300484

NCI-H3122 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)