IHH-4 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305448

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The IHH-4 cell line is derived from papillary thyroid carcinoma (PTC), the most prevalent form of thyroid cancer, which frequently exhibits aggressive characteristics including invasion and metastasis. IHH-4 has been utilized in numerous studies focused on elucidating the molecular mechanisms underpinning PTC progression. This cell line is particularly noted for its role in studies investigating epithelial-mesenchymal transition (EMT), a process that enhances the invasive potential of cancer cells. For example, it has been shown that IHH-4 cells, along with other PTC lines, express elevated levels of matrix metalloproteinase-9 (MMP-9), a protease that plays a critical role in degrading the extracellular matrix and facilitating tumor invasion and metastasis. Inhibition of MMP-9 in IHH-4 cells was found to reduce EMT markers and hinder cell migration and invasion. Research involving the IHH-4 cell line has also examined the role of transcription factors such as T cell factor 4 (TCF4) and long non-coding RNAs (lncRNAs) in PTC. Studies have highlighted that TCF4 is overexpressed in IHH-4 cells and can regulate the expression of the lncRNA HCP5, which in turn modulates several microRNAs related to tumor progression. Knockdown of TCF4 in IHH-4 cells was shown to decrease cell proliferation and invasion, suggesting that TCF4 is a pivotal regulator of oncogenic pathways in PTC. Overall, IHH-4 serves as a valuable model for studying molecular and cellular pathways related to thyroid cancer, particularly those that drive cancer cell invasion, metastasis, and resistance to therapies. The insights gained from research using IHH-4 contribute to the development of potential therapeutic strategies for combating aggressive thyroid cancers.
Organismo	Human
Tejido	Thyroid gland
Enfermedad	Thyroid gland papillary carcinoma
Lugar de metástasis	Left cervical lymph node
Sinónimos	IHH4

Edad	75 years
Género	Male
Origen étnico	Japanese
Morfología	Epithelial-like
Propiedades de crecimiento	Adherent

Referencia	IHH-4 (Cytion catalog number 305448)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_2960
Estado de los OGM	GMO-S1: This human thyroid papillary carcinoma cell line (IHH-4) contains undefined stable modifications consistent with tumor-derived immortalization. No infectious virus is produced. This classification applies only within Germany and may differ elsewhere.

Perfil mutacional	Mutation: AKT1, p.Glu17Lys (c.49G>A), heterozygous; Mutation: BRAF, p.Val600Glu (c.1799T>A), heterozygous; Mutation: CREBBP, p.Trp592Ter (c.1776G>A), heterozygous; Mutation: CRLF2, p.Trp255Ter (c.765G>A), heterozygous; Mutation: EP300, p.Arg1312Ter (c.3934C>T), heterozygous; Mutation: RAC1, p.Asp11Glu (c.33C>G), heterozygous; Mutation: TERT, c.1-124C>T (c.228C>T) (C228T), heterozygous

Medio de cultivo	1 to 1 mixture of Dulbecco's modified Eagle's medium (Cytion article number 820300a) and RPMI1640 medium (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% heat-inactivated FBS
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305448-301025	Certificado de análisis	05. Dec. 2025	305448

IHH-4 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)