IGROV-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305556

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The IGROV-1 cell line is a human ovarian adenocarcinoma cell line extensively utilized in research, particularly in studies involving ovarian cancer. Derived from an ovarian carcinoma, IGROV-1 cells are known for their utility in modeling epithelial ovarian cancer (EOC), which accounts for a majority of ovarian malignancies. This cell line has been employed in various contexts, including evaluating drug responses and mechanisms underlying drug resistance. For example, IGROV-1 has been instrumental in testing the efficacy of targeted therapies, such as the folate receptor alpha-targeting antibody-drug conjugate mirvetuximab soravtansine (IMGN853). This ADC showed promising results by synergizing with chemotherapeutics like carboplatin and doxorubicin, enhancing antitumor efficacy through DNA damage and cell cycle arrest in preclinical models. In addition to its role in cancer research, IGROV-1 has been characterized as a model for viral infection studies. Recent work highlighted its susceptibility to SARS-CoV-2, leveraging its expression of ACE2 to support viral replication. IGROV-1 was shown to mount a robust innate immune response upon infection, similar to primary human nasal epithelial cells, indicating its potential for serological assays, antiviral drug testing, and isolation of viral variants from patient samples. This cell line is considered advantageous for research due to its effective replication of viruses compared to traditional models like Vero cells, which can lead to adaptive mutations. Overall, IGROV-1 cells serve as a valuable model in both oncology and virology, supporting studies of tumor biology, drug resistance, and viral pathogenesis. Their relevance in drug synergy experiments and their compatibility with antiviral research underscore their versatility and importance in the field.
Organismo	Human
Tejido	Ovary
Enfermedad	Endometrioid carcinoma
Sinónimos	Igrov-1, IGROV 1, IGR-OV1, IGROV1, Igrov1, IGR.OV1, IGROV, OV1/P, OV1/p, OV1-P

Edad	47 years
Género	Female
Origen étnico	Caucasian
Morfología	Epithelial-like
Propiedades de crecimiento	Adherent, monolayer

Referencia	IGROV-1 (Cytion catalog number 305556)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_1304

Tumorigénico	Yes, in nude mice.
Perfil mutacional	Mutation: BRCA1, p.Lys654Serfs47 (c.1961delA), heterozygous; Mutation: BRCA2, p.Lys1108Argfs11 (c.3323delA) (p.Gln1107fs) (c.3320delA); Mutation: PIK3CA, p.Arg38Cys (c.112C>T), heterozygous; Mutation: PIK3CA, p.Ter1069TrpinsLysAspAsn (c.3207A>G), heterozygous; Mutation: PTEN, p.Thr319fs1 (c.955_958delACTT) (p.VL317fs) (V317fs3), heterozygous; Mutation: RB1, p.Val654Cysfs4 (c.1959delA), heterozygous; Mutation: SMAD4, p.Gly231Alafs10 (c.692delG), heterozygous; Mutation: SMAD4, p.Leu495Pro (c.1484T>C), heterozygous; Mutation: TP53, p.Ser90Leufs*59 (c.267dupC) (c.267_268insC), heterozygous; Mutation: TP53, p.Tyr126Cys (c.377A>G), heterozygous

Medio de cultivo	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suplementos	Supplement the medium with 10% FBS
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with TrypLE Express, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305556-150425	Certificado de análisis	21. Jul. 2025	305556

IGROV-1 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)