HSC-3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305312

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	HSC-3 is a human oral squamous cell carcinoma (OSCC) cell line commonly used to investigate oral cancer biology, particularly in studies focusing on apoptosis, cell cycle regulation, and cancer treatment. Oral squamous cell carcinoma is the most common type of oral cancer and is associated with poor prognosis due to its high metastatic potential and late-stage diagnosis. HSC-3 cells are derived from a primary tumor and are known for their aggressive properties, making them a relevant model for testing novel anticancer compounds and therapies. Several studies have demonstrated that HSC-3 cells undergo apoptosis and autophagy in response to natural compounds and anticancer agents. For example, piperine, an alkaloid from black pepper, was found to reduce cell viability and induce apoptosis in a dose-dependent manner. Apoptotic bodies, DNA fragmentation, and increased expression of pro-apoptotic proteins such as Bax were observed in HSC-3 cells treated with piperine. Additionally, piperine was shown to activate both apoptosis and autophagy through inhibition of the PI3K/Akt/mTOR signaling pathway, which is critical for cancer cell proliferation and survival. Similarly, other compounds like berberine and geniposide have also been shown to induce apoptosis by disrupting mitochondrial membrane potential and activating caspase pathways. The utility of HSC-3 cells extends to in vivo studies, where their use in mouse xenograft models has demonstrated tumor growth inhibition when treated with natural compounds like piperine. These cells serve as a robust platform for evaluating the effectiveness of both traditional and novel cancer therapies.
Organismo	Human
Tejido	Tongue
Enfermedad	Squamous cell carcinoma
Lugar de metástasis	Cervical lymph node
Sinónimos	HSC 3, HSC3

Edad	64 years
Género	Male
Origen étnico	Japanese
Propiedades de crecimiento	Adherent

Referencia	HSC-3 (Cytion catalog number 305312)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_1288

Perfil mutacional	Mutation: CDKN2A, p.Glu120Ter (c.358G>T), homozygous; Mutation: PIK3CA, p.Glu545Gly (c.1634A>G); Mutation: TERT, c.1-124C>T (c.228C>T); Mutation: TP53, p.Lys305fs (c.912_913insTAAG)

Medio de cultivo	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305312-120924	Certificado de análisis	22. Oct. 2025	305312
305312-150925	Certificado de análisis	05. Dec. 2025	305312

HSC-3 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)