UM-SCC-127 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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cryovial

Product number: 305725

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	UM-SCC-127 is a human HNSCC cell line from the University of Michigan UM-SCC panel. As part of the higher-numbered UM-SCC series, it represents one of the more recently established lines in this collection. The line grows adherently with epithelial-like morphology and provides a model for comparative molecular and pharmacological HNSCC studies. Applicable in HNSCC research, drug sensitivity studies, molecular pathway analysis, and UM-SCC multi-line panel experiments. Suitable for pharmacological assays and xenograft models.
Organism	Human
Tissue	Head and neck, oral cavity
Disease	Head and neck squamous cell carcinoma (HNSCC)
Applications	HNSCC research; drug sensitivity; molecular pathway analysis; UM-SCC panel studies; xenograft models

Age	Age unspecified
Gender	Sex unspecified
Ethnicity	Ethnicity unspecified
Morphology	Epithelial-like
Cell type	Epithelial cells
Growth properties	Adherent

Citation	UM-SCC-127 (Cytion catalog number 305725)
Biosafety level	1
NCBI_TaxID	9606

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase, 5 min., 37°C
Doubling time	approx. 24 to 48 hours
Subculturing	Remove medium, wash with PBS without calcium and magnesium, cover with Accutase, incubate 8–10 min at RT, resuspend in medium, centrifuge 300×g 3 min, discard supernatant, reseed in fresh medium.
Split ratio	1 to 5
Seeding density	1 to 3 × 10⁴ cells/cm²
Fluid renewal	Every 2 to 3 days
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Lot Number	Certificate Type	Date	Catalog Number
305725-030626	Certificate of Analysis	23. Jul. 2026	305725

UM-SCC-127 Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)