RM-1-Luc Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305703

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	RM-1-Luc is a bioluminescent derivative of the murine RM-1 prostate carcinoma cell line, engineered to stably express a firefly luciferase reporter gene. The parental RM-1 cell line was established from a carcinoma of the mouse prostate gland derived from fetal C57BL/6 tissue at 17 fetal days and is a rapidly growing, androgen-independent prostate cancer model in the C57BL/6 syngeneic background. RM-1 cells exhibit epithelial morphology, express typical prostate carcinoma markers, and form aggressive tumors in immunocompetent C57BL/6 hosts, making this model suitable for studying tumor-immune interactions and evaluating immunotherapy strategies against prostate cancer. The stable luciferase integration in RM-1-Luc enables sensitive, noninvasive bioluminescence imaging (BLI) of primary tumor growth and metastatic dissemination in living C57BL/6 mice following luciferin administration. The emitted signal correlates with viable tumor cell number, supporting longitudinal assessment of tumor engraftment, growth kinetics, and therapeutic response without repeated invasive procedures. RM-1-Luc is particularly valuable for preclinical evaluation of checkpoint inhibitors, androgen-deprivation strategies, and combination immunotherapy approaches in an immunocompetent prostate cancer model. RM-1-Luc retains the key biological features of the parental RM-1 line, including its androgen-independent growth, C57BL/6 syngeneic compatibility, and established use in prostate cancer research. The luciferase reporter enhances experimental sensitivity and enables real-time pharmacodynamic assessment. Researchers should validate luciferase activity, growth kinetics, and immunological phenotype under their specific experimental conditions prior to large-scale in vivo use.
Organism	Mouse
Tissue	Prostate
Disease	Carcinoma prostate gland
Synonyms	RM1

Breed/Subspecies	C57BL/6
Age	17 fetal days
Gender	Male
Morphology	Epithelial
Growth properties	Adherent

Citation	RM-1-Luc (Cytion catalog number 305703)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_E3IK
GMO Status	GMO-S1: This cell line contains a stably integrated firefly luciferase reporter cassette (Luc2, codon-optimized) introduced via replication-incompetent lentiviral transduction. The resulting polyclonal cell population was maintained under puromycin selection (1–5 µg/mL). S1 containment is required. This classification applies only within Germany and may differ elsewhere.

Antigen expression	Luc2 (firefly, codon-optimized)

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase 5 min, RT
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1 to 3
Seeding density	1 to 4 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Lot Number	Certificate Type	Date	Catalog Number
305703-120526	Certificate of Analysis	24. Jun. 2026	305703

RM-1-Luc Cells

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Certificate of Analysis (CoA)