Novikoff Hepatoma Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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cryovial

Product number: 500373

Safety data sheet

Product sheet

Description	Novikoff-Hepatoma, also known as Novikoff Hepatoma or NK, is a rat hepatocellular carcinoma cell line derived from a male Sprague Dawley rat (Rattus norvegicus). The tumor originated as an experimentally induced hepatoma and has been widely used as a transplantable and in vitro model of rat liver cancer. It represents a poorly differentiated hepatocellular carcinoma and is characterized by rapid proliferation and high tumorigenic capacity in syngeneic hosts. The N1-S1 cell line originates from the same individual tumor, indicating a shared genetic background between these related derivatives. Novikoff-Hepatoma cells exhibit morphological and biochemical features consistent with malignant hepatocytes, including altered metabolic activity, dysregulated cell cycle control, and enhanced nucleolar and ribosomal biogenesis typical of rapidly growing hepatic tumors. Historically, this model has been extensively used in studies of liver carcinogenesis, tumor metabolism, RNA and protein synthesis, and chemotherapeutic response in rodent systems. Due to its robust growth characteristics and reproducibility, the line has served as a classical model in experimental oncology, particularly for investigating hepatocellular carcinoma biology in immunocompetent rat models. As a Sprague Dawley - derived tumor line, Novikoff-Hepatoma is compatible with syngeneic transplantation studies in the corresponding rat strain, enabling investigation of tumor-host interactions, therapeutic interventions, and loco-regional treatment strategies such as intra-arterial drug delivery. Its well-documented experimental history and stable malignant phenotype make it a valuable preclinical model for mechanistic studies of hepatocellular carcinoma progression and treatment response in vivo and in vitro.
Organism	Rat
Tissue	Liver
Disease	Hepatocellular carcinoma
Applications	Induction of hepatoma
Synonyms	Novikoff-Hepatoma, NK

Breed/Subspecies	Sprague-Dawley
Gender	Male
Growth properties	Suspension, some adherent cells

Citation	Novikoff Hepatoma (Cytion catalog number 500373)
Biosafety level	1
NCBI_TaxID	10116
CellosaurusAccession	CVCL_1D01

Tumorigenic	Yes, in Sprague-Dawley Rat

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Subculturing	Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10⁵ cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
Seeding density	1 x 10⁵ cells/ml
Post-Thaw Recovery	Good. Allow the cells to recover from the freezing process for at least 24 to 48 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Novikoff Hepatoma Cells

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