NCI-H1734 Cells

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305833

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	NCI-H1734 is a human non-small cell lung cancer (NSCLC) cell line derived from a lung adenocarcinoma. It was established as part of the extensive NCI cell line program focused on thoracic malignancies and has been utilized in a broad range of preclinical studies related to lung cancer biology and therapeutic response. This cell line harbors a KRAS mutation, a characteristic that aligns with a subset of NSCLC cases known to exhibit resistance to EGFR inhibitors and altered responses to MEK and PI3K pathway-targeted therapies. Due to its molecular profile, NCI-H1734 is often used to model KRAS-driven tumorigenesis and to test compounds targeting downstream signaling cascades. Protein expression analyses, including reverse-phase protein arrays (RPPA), have revealed that NCI-H1734 exhibits a distinct phosphorylation pattern indicative of active PI3K/AKT and MAPK signaling. This has made it a valuable system for exploring oncogenic signaling dynamics and drug responsiveness. The cell line demonstrates sensitivity to agents targeting components of these pathways, including MEK inhibitors, and serves as a useful comparator in panels assessing pathway dependency across KRAS-mutant NSCLC models. Additionally, it is included in comprehensive molecular datasets such as the Cancer Cell Line Encyclopedia and has been evaluated in large-scale drug screening efforts to correlate proteomic and genomic features with pharmacologic profiles. Histologically, NCI-H1734 cells exhibit characteristics of glandular epithelial origin, and they grow in adherent monolayers in vitro. They have been included in studies that investigate the impact of tumor microenvironment interactions and epithelial-mesenchymal transition (EMT) on therapeutic resistance. The inclusion of NCI-H1734 in several profiling studies has enhanced its utility as a reference model for adenocarcinoma subtypes within NSCLC, contributing to the development of precision oncology strategies for KRAS-mutant lung cancers.
Organism	Human
Tissue	Lung
Disease	Lung adenocarcinoma
Synonyms	H1734, H-1734, NCIH1734

Age	56 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial
Growth properties	Adherent/Suspension

Citation	NCI-H1734 (Cytion catalog number 305833)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1491

Mutational profile	Mutation: ATM, Simple, p.Gln65Pro (c.194A>C), Heterozygous, KRAS, Simple, p.Gly13Cys (c.37G>T), Heterozygous, RB1, Simple, p.Ser127Ile (c.380G>T), Homozygous, TP53, Simple, p.Arg273Leu (c.818G>T), Homozygous

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Seeding density	3-5*10^4/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

The cells are sensitive to full medium changes and tend to detach afterwards. We therefore recommend complete medium changes only on Mondays and Fridays, and simply adding medium on the remaining days.
Centrifuge at 100 × g for 8 minutes.
Fresh medium for subcultivation must be pre-equilibrated at 37 °C and 5% CO₂ to avoid pH shifts, which can trigger apoptosis.
Maximum confluence: 60%.