MKN1 Cells
CAD$759.00*
Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 106 cells for adherent lines or 5 × 106 cells for suspension lines (refer to the batch CoA for details).
General information
| Description | MKN1 is a human gastric cancer cell line derived from an adult patient with poorly differentiated adenocarcinoma. This cell line is widely used in cancer research due to its relevance in modeling gastric cancer progression, invasion, and response to therapies. It exhibits a mesenchymal-like morphology, indicative of epithelial-to-mesenchymal transition (EMT), a process critical in cancer metastasis. The MKN1 cell line is notable for its moderate to low expression of key proteins like RhoA, making it an essential tool in studying signaling pathways linked to cell adhesion and motility. Recent research highlights the importance of MKN1 in exploring the role of DCLK1 (Doublecortin-like kinase 1), a putative cancer stem cell marker, in gastric cancer. Overexpression of DCLK1 in MKN1 cells promotes EMT and enhances the secretion of small extracellular vesicles (sEVs), which are implicated in cell migration and tumor microenvironment remodeling. Proteomic analysis of sEVs from DCLK1-overexpressing MKN1 cells has identified significant alterations in proteins involved in migration and adhesion, such as STRAP and BCAM. Furthermore, DCLK1's kinase activity appears to regulate the selection of cargo proteins within these vesicles, underscoring its potential as a therapeutic target. Additionally, studies involving the RHOA mutation (R129W) in MKN1 cells have revealed its role in altering cell migration and adhesion pathways, independent of CDH1 mutations. Functional assays show that the mutation results in a higher active GTP-bound state of RhoA, linking it to enhanced cell motility. These findings position MKN1 as a robust model for dissecting genetic and molecular mechanisms underlying gastric cancer pathogenesis and testing potential therapeutic interventions. |
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| Organism | Human |
| Tissue | Stomach |
| Disease | Adenosquamous carcinoma |
| Metastatic site | Lymph node |
| Synonyms | MKN-1, MKN 1 |
Characteristics
| Age | 72 years |
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| Gender | Male |
| Ethnicity | Japanese |
| Morphology | Epithelial-like |
| Growth properties | Adherent |
Regulatory Data
| Citation | MKN1 (Cytion catalog number 305589) |
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| Biosafety level | 1 |
| NCBI_TaxID | 9606 |
| CellosaurusAccession | CVCL_1415 |
Biomolecular Data
| Mutational profile | Mutation: TP53, p.Val143Ala (c.428T>C) |
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Handling
| Culture Medium | RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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| Supplements | Supplement the medium with 10% FBS |
| Dissociation Reagent | Accutase |
| Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with TrypLE Express, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
| Freeze medium | As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
| Thawing and Culturing Cells |
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| Incubation Atmosphere | 37°C, 5% CO2, humidified atmosphere. |
| Shipping Conditions | Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage. |
| Storage Conditions | For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen. |
Quality Control & Molecular Analysis
| Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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