MHCC-97H-Luc Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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cryovial

Product number: 305687

Safety data sheet

Product sheet

Description	MHCC-97H-Luc is a bioluminescent derivative of the human MHCC-97H hepatocellular carcinoma cell line, engineered to stably express a firefly luciferase reporter gene. The parental MHCC-97H cell line was established from a hepatocellular carcinoma in a 39-year-old Chinese male patient and is characterized by high metastatic potential, as reflected by its designation as the high-metastasis subline of the MHCC97 series. MHCC-97H cells are associated with hepatitis B virus (HBV) transformation and exhibit aggressive invasive behavior, making them a clinically relevant model for studying highly metastatic hepatocellular carcinoma and therapeutic strategies targeting invasion and dissemination. The stable luciferase integration in MHCC-97H-Luc enables sensitive, noninvasive bioluminescence imaging (BLI) of tumor burden in orthotopic liver implantation and subcutaneous xenograft models using immunocompromised hosts. The emitted signal correlates with viable tumor cell number, supporting longitudinal monitoring of tumor engraftment, intrahepatic spread, and distant metastasis. MHCC-97H-Luc is particularly valuable for in vivo evaluation of anti-metastatic agents, kinase inhibitors, and novel targeted therapies against highly invasive hepatocellular carcinoma, as well as for investigating tumor microenvironment interactions and molecular mechanisms underlying HCC metastasis. MHCC-97H-Luc retains the high metastatic potential and HBV-associated molecular features of the parental MHCC-97H line, providing a relevant and aggressive preclinical platform for hepatocellular carcinoma research. The luciferase reporter enhances experimental sensitivity and enables real-time pharmacodynamic assessment. Researchers should validate luciferase activity, metastatic behavior, and growth kinetics under their specific experimental conditions prior to large-scale in vivo use.
Organism	Human
Tissue	Liver
Disease	Adult hepatocellular carcinoma
Synonyms	MHCC 97-H, MHCC97-H, MHCC97H

Age	39 years
Gender	Male
Ethnicity	Chinese
Morphology	Epithelial-like
Cell type	Hepatocyte-like
Growth properties	Adherent

Citation	MHCC-97H-Luc (Cytion catalog number 305687)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_4972

Antigen expression	Luc2 (firefly, codon-optimized)
Tumorigenic	High metastatic potential
Viruses	Transformant: Hepatitis B virus (HBV)

Culture Medium	DMEM
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1 to 3
Seeding density	1 to 3 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

MHCC-97H-Luc Cells

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