HT55 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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CAD$593.40*

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cryovial

Product number: 305018

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	HT55 is a human rectal adenocarcinoma cell line established from the primary rectal tumor of a 54-year-old Caucasian female. The line grows as an adherent monolayer with epithelial-like morphology. HT55 carries multiple heterozygous and homozygous loss-of-function mutations in the APC (adenomatous polyposis coli) tumor suppressor gene, including p.Gln1131Ter, p.Gln1303Ter, p.Arg1463Ser, p.Asn581Tyr, and p.Lys241Ter (all heterozygous), as well as a homozygous p.Arg213Leu substitution. This mutational profile renders HT55 a biologically relevant model of APC-driven colorectal carcinogenesis, reflecting the canonical WNT pathway dysregulation that drives the majority of human colorectal cancers. HT55 is widely used in colorectal cancer research for studies of WNT/β-catenin signalling, APC tumor suppressor function, drug sensitivity and resistance, and preclinical evaluation of targeted agents against components of the WNT pathway. The line is suitable for in vitro pharmacological assays and xenograft studies in immunocompromised mouse models, providing an adherent, tractable in vitro platform for mechanistic and translational colorectal cancer research. The presence of multiple APC truncation mutations makes HT55 representative of the microsatellite-stable, chromosomally unstable colorectal cancer subtype commonly driven by APC loss-of-function.
Organism	Human
Tissue	Rectum
Disease	Adenocarcinoma
Applications	Colorectal cancer research; WNT/APC pathway studies; drug sensitivity and resistance testing; preclinical oncology; xenograft models; targeted therapy evaluation
Synonyms	HT55

Age	54 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Cell type	Epithelial cells
Growth properties	Adherent

Citation	HT55 (Cytion catalog number 305018)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1294

Mutational profile	Mutation: p.Gln1131Ter, Heterozygous; Mutation: p.Gln1303Ter, Heterozygous; Mutation: p.Arg1463Ser, Heterozygous; Mutation: p.Asn581Tyr, Heterozygous; Mutation: p.Lys241Ter, Heterozygous; Mutation: p.Arg213Leu, Homozygous

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	28 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300×g for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1 to 5
Seeding density	1 to 3 x 10⁴ cells/cm²
Fluid renewal	Every 2 to 3 days
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Lot Number	Certificate Type	Date	Catalog Number
305018-240426	Certificate of Analysis	23. Jul. 2026	305018

HT55 Cells

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Certificate of Analysis (CoA)