CTX Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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cryovial

Product number: 305358

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	CTX (also designated CTX0E03) is a conditionally immortalized human neural stem cell line derived from first-trimester human fetal cortical neuroepithelium. The line was generated by retroviral integration of a c-mycER^TAM fusion transgene, which drives cell proliferation only in the presence of 4-hydroxytamoxifen (4-OHT). In the absence of 4-OHT and in mitogen-free medium, CTX0E03 cells undergo growth arrest and differentiate into neurons and astrocytes, retaining multipotent differentiation capacity. The line is clonal, has a normal human karyotype (46,XY), and was developed under GMP-compatible conditions for potential clinical application. CTX0E03 has been evaluated in preclinical models of ischemic stroke (MCAo rat model), where implanted cells promoted significant recovery in sensorimotor function. The line has entered clinical trials for stroke therapy. It is also used as an in vitro model for neural stem cell biology, cortical neuron differentiation, Alzheimer’s disease research (Abeta toxicity studies), and neuropharmacological screening. The conditional immortalization system makes CTX0E03 particularly suitable for safety and efficacy studies, as proliferation can be switched off by removal of 4-OHT prior to transplantation.
Organism	Human
Tissue	Brain (cerebral cortex)
Disease	Normal fetal cortical neuroepithelium; conditionally immortalized neural stem cell line
Applications	Neural stem cell biology; cortical neuron differentiation; stroke research; neurodegenerative disease modeling; Alzheimer’s disease research; neuropharmacological screening; cell therapy research

Morphology	Neural stem cell-like (bipolar, elongated)
Cell type	Neural stem cells
Growth properties	Adherent (on laminin-coated surfaces)

Citation	CTX (Cytion catalog number 305358)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_C2BI
GMO Status	GMO-S1: Contains a single-copy retroviral c-mycERTAM conditional immortalization transgene. Proliferation is 4-OHT-dependent. Not tumorigenic in vivo. This classification applies within Germany; regulations may differ in other jurisdictions.

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase, 5 min., 37°C
Doubling time	50 to 60 hours
Subculturing	When 70–80% confluent, aspirate medium, wash with PBS (Ca²⁺/Mg²⁺-free), add Accutase and incubate 5–10 min at 37°C. Neutralize with complete medium, centrifuge at 300 × g for 5 min, resuspend in fresh medium with 4-OHT, and replate on pre-coated flasks. Coat flasks with laminin (10 μg/ml in PBS) 1 h at 37°C prior to plating.
Split ratio	1 to 3
Seeding density	1 to 3 × 10⁴ cells/cm²
Fluid renewal	Every 3 to 5 days (depending on confluency)
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.

Lot Number	Certificate Type	Date	Catalog Number
305358-180526	Certificate of Analysis	03. Jul. 2026	305358

CTX Cells

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Certificate of Analysis (CoA)