Y-79 Cells
Product number:
300382
General information
| Description | The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation. The donor had a strong maternal family history of retinoblastoma. Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor. |
|---|---|
| Organism | Human |
| Tissue | Retina |
| Disease | Retinoblastoma |
| Synonyms | Y79, GM01232, GM01232E |
Characteristics
| Age | 2,5 years |
|---|---|
| Gender | Female |
| Ethnicity | Caucasian |
| Morphology | Multicellular clusters |
| Growth properties | Clusters in Suspension |
Regulatory Data
| Citation | Y-79 (Cytion catalog number 300382) |
|---|---|
| Biosafety level | 1 |
| NCBI_TaxID | 9606 |
| CellosaurusAccession | CVCL_1893 |
Biomolecular Data
| Isoenzymes | PGM1, 1, G6PD, B, ES-D, 1, AK-1, 1, GLO-1, 2, Phenotype Frequency Product: 0.1373 |
|---|---|
| Reverse transcriptase | Negative |
| Karyotype | Hypertriploid, with abnormalities including dicentrics, breaks, pulverizations and minutes |
Handling
| Culture Medium | RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
|---|---|
| Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
| Split ratio | A ratio of 1:2 to 1:4 is recommended |
| Seeding density | 1 x 10^5 cells/ml |
| Fluid renewal | Every 3 days |
| Freeze medium | As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
Quality Control & Molecular Analysis
| Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
|---|---|
| STR profile |
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13,14
D5S818: 11,12
D7S820: 8,9
TH01: 6,9.3
TPOX: 8
vWA: 15,18
D3S1358: 15,16
D21S11: 30,32
D18S51: 13,16
Penta E: 13,18
Penta D: 12
D8S1179: 13,16
FGA: 22
|
| HLA alleles |
A*: '02:01:01
B*: '40:01:02, '51:01:01
C*: '03:04:01, '12:03:01
DRB1*: '01:01:01, '13:02:01
DQA1*: '01:01:01, '01:02:01
DQB1*: '05:01:01, '06:04:01
DPB1*: '03:01:01, '04:01:01
E: '01:01, '01:03
|
Certificate of Analysis (CoA)
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 300382-150725 | Certificate of Analysis | 27. Mar. 2026 | 300382 |
| 300382-516SF | Certificate of Analysis | 18. Aug. 2025 | 300382 |