U-CH1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$690.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305885

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The U-CH1 cell line is the first established permanent human chordoma cell model, derived from a recurrent sacral chordoma. Chordomas are rare, slow-growing, locally invasive tumors that originate from notochordal remnants and primarily occur along the axial skeleton. U-CH1 exhibits cytogenetic features characteristic of chordoma, including clonal chromosomal aberrations such as der(1)t(1;22), deletions on chromosomes 4, 5, 6, 9, 10, and 20, and a derivative chromosome 20 resulting from t(10;20). Comparative genomic hybridization revealed recurrent DNA copy number changes in chordomas, particularly losses on 1p and 3p and gains on 7q, 5q, 12q, and 20. The cytogenetic profile of U-CH1 closely mirrors that of its parental tumor, reinforcing its biological relevance. Functionally and molecularly, U-CH1 and other chordoma cell lines display hallmark features of chordoma, including expression of brachyury, a transcription factor considered a key diagnostic marker. U-CH1 also harbors deletions of CDKN2A and lacks p16 protein expression, a recurrent genetic alteration in chordomas. This alteration leads to hyperactivation of the CDK4/6 pathway, rendering U-CH1 sensitive to CDK4/6 inhibitors such as palbociclib. Treatment with palbociclib significantly reduced phosphorylated Rb levels and inhibited proliferation in vitro, indicating that U-CH1 can be a valuable preclinical model for evaluating cell cycle-targeted therapies. The cell line has also been validated through mRNA and protein profiling, confirming its representativeness of primary chordoma tumors in expression and genomic patterns.
Organism	Human
Tissue	Bone, sacrum
Disease	Sacral chordoma
Synonyms	UCH-1, UCH1

Age	56 years
Gender	Male
Ethnicity	White
Morphology	Mesenchymal like, with variable vacuoles
Cell type	Chordoma
Growth properties	Adherent

Citation	U-CH1 (Cytion catalog number 305885)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_4988

Mutational profile	Mutation: TP53, Simple, p.Pro72Arg (c.215C>G), Unspecified

Culture Medium	IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	~1 week
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305885-020126	Certificate of Analysis	03. Feb. 2026	305885

U-CH1 Cells

General information

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Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)