SW620-Luc Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305704

Safety data sheet

Product sheet

Organism	Human
Tissue	Metastatic
Disease	Colon adenocarcinoma
Metastatic site	Lymph node
Synonyms	SW620, SW 620, SW.620

Age	51 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Adherent

Citation	SW620-Luc (Cytion catalog number 305704)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_J268
GMO Status	GMO-S1: This SW-620 line also contains a-Luc cassette enabling bioluminescent monitoring of metastatic progression. This classification applies only within Germany and may differ elsewhere.

Protein expression	Luc
Tumorigenic	Yes, in athymic nude mice
Mutational profile	Mutation: p.Gln1338Ter, Homozygous; Mutation: p.Gly12Val, Homozygous; Mutation: p.Arg273His, Heterozygous; Mutation: p.Pro309Ser, Heterozygous

Culture Medium	DMEM
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Properties "SW620-Luc Cells"

Metastatic site:	Lymph node

SW620-Luc Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis