RBL-2H3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305194

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The RBL-2H3 cell line has become a valuable tool for studying mast cell physiology. RBL-2H3 cells express rat mast cell protease II (RMCP-II) and the c-kit receptor tyrosine kinase, making them a potential model for mast cells. However, conflicting and sometimes misleading data about RBL-2H3 cells have been reported. RBL-2H3 cells have been widely used to investigate various aspects of mast cell function, including degranulation, mast cell stabilizers, and the interaction of FceRI receptors with the cytoskeleton. They express high-affinity IgE receptors and can be activated to secrete histamine and other mediators. Cultivating RBL-2H3 cells is relatively easy, and longer culturing times result in higher cell density. Degranulation is a key feature of RBL-2H3 cells, similar to mast cells and basophils. When allergens crosslink their IgE-bound FceRI receptors, RBL-2H3 cells release preformed and newly synthesized mediators, contributing to immune allergic responses. The degranulation of RBL-2H3 cells has provided insights into basophil degranulation as well. These cells can also undergo degranulation in response to non-immunological stimuli, and there are differences between MMC, RBL-2H3, and CTMC. The role of calcium in RBL-2H3 cell degranulation is significant. The calcium ionophore A23187, which increases intracellular calcium levels, induces degranulation in RBL-2H3 cells, similar to mast cells and basophils. Some studies have described RBL-2H3 cells as a serotonin-releasing cell line.
Organism	Rat
Tissue	Peripheral blood
Disease	Rat leukemia
Synonyms	RBL2H3, RBL 2H3, RBL.2H3

Breed/Subspecies	Wistar
Morphology	Fibroblast
Growth properties	Adherent

Citation	RBL-2H3 (Cytion catalog number 305194)
Biosafety level	1
NCBI_TaxID	10116
CellosaurusAccession	CVCL_0591

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305194-120124	Certificate of Analysis	23. May. 2025	305194
305194-300625	Certificate of Analysis	22. Oct. 2025	305194
305194-041022	Certificate of Analysis	23. May. 2025	305194

RBL-2H3 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)