Neuro2a-Luc Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305690

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	Neuro-2a-Luc is a luciferase-expressing derivative of the Neuro-2a (N2a) mouse neuroblastoma cell line. Neuro-2a cells originate from murine neural crest-derived neuroblastoma tissue and are widely used as an in vitro model for neuronal differentiation, neurotoxicity studies, signal transduction research, and neuro-oncology investigations. Stable expression of a luciferase reporter enables sensitive, quantitative bioluminescent detection of viable cells and cellular activity, making Neuro-2a-Luc particularly useful for longitudinal monitoring in both in vitro and in vivo experimental systems. Depending on the reporter design, luciferase expression may be constitutive or linked to pathway-specific promoter activity. Neuro-2a-Luc cells are commonly employed in applications involving tumor growth tracking, high-throughput drug screening, neural differentiation assays, and real-time assessment of therapeutic responses. In xenograft and metastasis models, luciferase-based bioluminescence imaging allows noninvasive monitoring of tumor burden and disease progression with high sensitivity. Neuro-2a-derived systems are also extensively used to study neuronal morphology, neurite outgrowth, apoptosis, oxidative stress, and mechanisms associated with neurodegenerative disease. The luciferase modification facilitates rapid quantitative analysis of cell proliferation, cytotoxicity, transcriptional activity, or pathway modulation in response to pharmacological or genetic perturbations. As with other engineered reporter cell lines, experimental performance of Neuro-2a-Luc may depend on factors including luciferase construct integration site, promoter configuration, substrate compatibility, and stability of reporter expression over serial passage. Additional characterization data, including details regarding the luciferase variant, selection marker, and validation assays, may be required for highly specialized experimental applications.
Organism	Mouse
Tissue	Peripheral nervous system
Disease	Neuroblastoma
Synonyms	Neuro2A-Luc

Gender	Male
Cell type	Neuronal and amoeboid stem cells
Growth properties	Adherent

Citation	Neuro-2a-Luc (Cytion catalog number 305690)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_K046

Protein expression	Luc
Antigen expression	H-2a
Viruses	Ectromelia virus (mousepox): negative
Virus resistance	Poliovirus 1
Reverse transcriptase	Negative
Products	Tubulin, acetylcholinesterase

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 to 3 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Lot Number	Certificate Type	Date	Catalog Number
305690-100426	Certificate of Analysis	15. May. 2026	305690

Neuro2a-Luc Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)