MRC-9 Cells
CAD$545.10*
Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 106 cells for adherent lines or 5 × 106 cells for suspension lines (refer to the batch CoA for details).
No longer available
General information
| Description | The MRC-9 cell line is a human diploid fibroblast strain derived from the lungs of a White female fetus at 15 weeks gestation. It was established for long-term use in biological research, vaccine production, and diagnostic virology. The cells exhibit adherent growth properties and maintain a stable, diploid karyotype throughout serial propagation. Importantly, MRC-9 cells possess normal chromosomal features and are non-tumorigenic under typical conditions. However, they have demonstrated tumorigenic properties when tested in semisolid media, but not when implanted in immunosuppressed mice. MRC-9 cells have been widely used for viral susceptibility studies, showing broad susceptibility to various viruses such as poliovirus, adenovirus, rubella, and cytomegalovirus. These cells are particularly valuable for their use in virology research and vaccine development. While their proliferation rate is slower compared to other human fibroblast strains like MRC-5, MRC-9 cells can still undergo a significant number of population doublings, with reports of up to 46 doublings. |
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| Organism | Human |
| Tissue | Fetal lung |
| Synonyms | MRC9, Medical Research Council cell strain-9 |
Characteristics
| Age | Fetus |
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| Gender | Female |
| Cell type | Fibroblast |
| Growth properties | Adherent |
Regulatory Data
| Citation | MRC-9 (Cytion catalog number 300394) |
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| Biosafety level | 1 |
| NCBI_TaxID | 9606 |
| CellosaurusAccession | CVCL_2629 |
Biomolecular Data
Handling
| Culture Medium | EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a) |
|---|---|
| Supplements | Supplement the medium with 10% FBS and 1% NEAA |
| Dissociation Reagent | Accutase |
| Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
| Freeze medium | As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
| Thawing and Culturing Cells |
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| Incubation Atmosphere | 37°C, 5% CO2, humidified atmosphere. |
| Shipping Conditions | Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage. |
| Storage Conditions | For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen. |
Quality Control & Molecular Analysis
| Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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Certificate of Analysis (CoA)
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 300394-070125 | Certificate of Analysis | 23. May. 2025 | 300394 |