MOLT-3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300116

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	MOLT-3 is a human T lymphoblast cell line derived from the peripheral blood of a 19-year-old male patient with acute lymphoblastic leukemia (ALL), specifically during a relapse following prior chemotherapy. This cell line was deposited by Dr. J. Minowada and is closely related to the MOLT-4 cell line, both originating from the same patient. MOLT-3 cells are widely used in research on immune system disorders, immunology, and immuno-oncology, making it an important model for studying T-cell leukemia and the immune response to various treatments. As a suspension cell line, MOLT-3 exhibits typical T-cell markers, including high expression of CD5 (97%) and CD7 (97%), along with CD1 and CD4. This cell line is also characterized by elevated terminal deoxynucleotidyl transferase (TdT) activity, which is commonly associated with immature lymphoid cells. MOLT-3 is valuable for studying T-cell differentiation, receptor signaling, and apoptosis, especially in the context of T-cell acute lymphoblastic leukemia (T-ALL). Due to its growth properties and well-characterized antigen expression, it is frequently utilized in drug screening and therapeutic research for leukemia treatments. Additionally, MOLT-3 cells do not produce immunoglobulins or contain detectable Epstein-Barr virus (EBV), which makes them an excellent model for studying T-cell-specific pathways without interference from B-cell characteristics. The cell line's response to various experimental manipulations further enhances its application in immuno-oncology, particularly for exploring potential therapeutic interventions targeting T-cell malignancies.
Organism	Human
Tissue	Peripheral blood
Disease	Acute lymphoblastic leukemia (ALL)
Synonyms	Molt-3, MOLT 3, Molt 3, MOLT3, Molt3

Age	19 years
Gender	Male
Ethnicity	Caucasian
Morphology	Round cells
Cell type	T lymphocyte
Growth properties	Suspension

Citation	MOLT-3 (Cytion catalog number 300116)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0624

Antigen expression	CD1(+), CD5(+), CD7(+), CD11a(+) (Greenberg et al. 1988).
Karyotype	Hypertetraploid

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with heat-inactivated 10% FBS
Doubling time	24 to 48 hours
Subculturing	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 10⁵ cells/ml and keep the cell concentration within the range of 3 x 10⁵ to 1 x 10⁶ cells/ml for optimal growth.
Seeding density	0.5 to 1 x 10⁵ cells/ml
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300116-230425	Certificate of Analysis	21. Jul. 2025	300116

MOLT-3 Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)