MIA PaCa-2 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300438

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The MIA PaCa-2 cell line is an indispensable asset in the field of cancer research and was derived from the pancreatic carcinoma tissue of a 65-year-old male. Mia PaCa 2 cells are widely used in the study of pancreatic ductal adenocarcinoma (PDAC), a notoriously aggressive and lethal cancer type. The cell line offer a solid tumor model that reflects the cellular characteristics of PDAC. One of the key attributes of this cell line is its genetic profile, which includes mutations in critical genes like KRAS and TP53, which are emblematic of the genetic landscape observed in pancreatic cancer patients. The cells have been extensively utilized to investigate various aspects of pancreatic cancer growth, metastasis, and resistance to therapeutics. Mia Paca-2 cells are instrumental in assessing the efficacy of chemotherapeutic drugs. Furthermore, the cell line serves as a vital resource for probing into the signaling pathways pivotal for cancer cell survival and metastasis, including the MAPK, PI3K/AKT, and Wnt pathways. Studies utilizing MIA PaCa-2 cells have also shed light on the dynamic interactions between cancer cells and their microenvironment. MIA PaCa-2's robust in vitro growth and its capacity to form tumors in xenograft models make it particularly suited for examining cancer progression and the mechanisms of tumorigenesis. In summary, the Mia Paca-2 cell line, with its broad application in pancreatic cancer research, continues to be a critical resource for scientists worldwide.
Organism	Human
Tissue	Pancreas
Disease	Ductal adenocarcinoma
Synonyms	MIA-PaCa-2, MIA-PACA-2, MIA-Pa-Ca-2, MIA Paca2, MIA PaCa2, MiaPaCa-2, MIAPACA-2, MiaPaca.2, MiaPaCa2, Miapaca2, MIAPaCa2, MIAPACA2, Mia PACA 2, MIAPaCa-2, PaCa2

Age	65 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Adherent with loosely attached rounded cells

Citation	MIA PaCa-2 (Cytion catalog number 300438)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0428

Isoenzymes	G6PD, B
Tumorigenic	Growth in soft agar. Formation of progressively growing carcinomas in nude athymic mice.
Mutational profile	Homozygous for KRAS p.Gly12Cys (c.34G>T) Homozygous for CDKN2A deletion
Karyotype	Hypotriploid

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	25 to 40 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 2 to 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300438-070725	Certificate of Analysis	18. Aug. 2025	300438
300438-280923	Certificate of Analysis	23. May. 2025	300438

MIA PaCa-2 Cells

Basic details about the Mia Paca-2 cell line

Aspects

Documentation

Genetic profile of the ductal adenocarcinoma cell line Mia Paca-2

Culturing methods

Purity and identity checks for the pancreatic cancer cell line Mia Paca 2

Certificate of Analysis (CoA)