MDA-MB-231-GFP

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305691

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	MDA-MB-231-GFP is a fluorescently labeled variant of the widely used MDA-MB-231 human breast cancer cell line, engineered to express green fluorescent protein (GFP) via lentiviral transduction. This modification enables real-time visualization and quantification of tumor cell dynamics both in vitro and in vivo, facilitating detailed analysis of tumor-stroma interactions, cellular proliferation, and metastatic behavior. The parental MDA-MB-231 line originates from a pleural effusion of a patient with triple-negative breast cancer (TNBC) and exhibits aggressive, invasive behavior with a mesenchymal phenotype, making it a cornerstone model for studying TNBC pathophysiology and treatment resistance. In co-culture experiments with human mesenchymal stem/stromal cells (MSCs), MDA-MB-231-GFP cells have demonstrated significantly enhanced proliferation and tumor-promoting behavior. Studies showed that direct contact with MSCs, rather than soluble factors alone, is critical for this effect. Specifically, co-culture with MSCs led to a 39.5% increase in MDA-MB-231-GFP cell proliferation after four days compared to monoculture, and induced expression of CD90 on a subset of breast cancer cells—a marker not expressed under standard conditions. This MSC-induced CD90 expression required direct cell-cell interaction and was partially inhibited by blocking gap junctions or Notch signaling, indicating the involvement of specific intercellular communication pathways. In vivo, co-injection of MDA-MB-231-GFP cells with MSCs into immunodeficient NOD/scid mice resulted in approximately tenfold increased tumor volume and enhanced metastatic potential compared to injection of cancer cells alone. These tumors exhibited elevated vascularization and higher viability, and retained a minority CD90-positive population, reinforcing the in vitro findings. Together, these studies position MDA-MB-231-GFP as a robust model for investigating tumor-stroma interactions, MSC-induced phenotypic plasticity, and mechanisms of tumor progression in triple-negative breast cancer.
Organism	Human
Tissue	Metastatic
Disease	Breast adenocarcinoma
Metastatic site	Pleural effusion

Age	51 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial
Growth properties	Adherent

Citation	MDA-MB-231-GFP (Cytion catalog number 305691)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_E2QK
GMO Status	GMO-S1: This MDA-MB-231 human breast carcinoma line contains a GFP construct for fluorescent monitoring of invasive behavior. This classification applies only within Germany and may differ elsewhere.

Protein expression	GFP
Mutational profile	Mutation: p.Gly464Val, Heterozygous; Mutation: p.Gly13Asp, Heterozygous; Mutation: p.Arg280Lys, Homozygous

Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion 820400a)
Supplements	Supplement the medium with 5% FBS
Dissociation Reagent	Accutase
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Properties "MDA-MB-231-GFP"

Metastatic site:	Pleural effusion

Lot Number	Certificate Type	Date	Catalog Number
305691-200825	Certificate of Analysis	22. Oct. 2025	305691

MDA-MB-231-GFP

General information

Characteristics

Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)