MA-Balb Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 400270

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	Ma-Balb is a mouse cell line established in vitro from a solid, spontaneously occurring mammary carcinoma in a female BALB/c mouse. This cell line originated from a solid, bean-sized tumor obtained from the mammary region of a young BALB/c mouse. Ma-Balb cells are significant in cancer research, particularly for studying mammary tumors, due to their derivation from a tumor-prone strain known for developing such cancers. The Ma-Balb cell line, with its fibroblast-like morphology, provides a robust model for investigating the cellular and molecular mechanisms driving mammary carcinoma. Researchers utilize these cells to explore the genetic and environmental factors that contribute to tumorigenesis, enabling a deeper understanding of cancer biology. Furthermore, Ma-Balb cells are instrumental in testing new anticancer treatments, offering insights into drug efficacy and toxicity. Their relevance extends to immunological studies, where they help elucidate the interactions between cancer cells and the immune system, thereby contributing to the advancement of immunotherapies.
Organism	Mouse
Tissue	Breast
Disease	Malignant neoplasms of the mouse mammary gland
Synonyms	Ma-Balb

Breed/Subspecies	BALB/c
Age	1 year
Gender	Female
Morphology	Epithelial-like
Cell type	Fibroblast
Growth properties	Adherent

Citation	MA-Balb (Cytion catalog number 400270)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_5795

Tumorigenic	Yes, in Balb/c mice
Viruses	MAP-test negative.

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	24 to 48 hours
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
400270-413	Certificate of Analysis	23. May. 2025	400270

MA-Balb Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)