KHM-5M Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305148

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The KHM-5M cell line is an important model derived from a patient with undifferentiated thyroid carcinoma complicated by neutrophilia and malignant pleurisy. This cell line is characterized by its significant production of neutrophil chemotactic factors, specifically human interleukin 8 (IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF). These factors are crucial in the recruitment and activation of neutrophils, which play a pivotal role in the immune response and inflammation. The KHM-5M cells were shown to possess extreme chemotactic activity, a trait that was substantiated through in vitro experiments using conditioned media from the cells and the modified Boyden chamber technique. Additionally, KHM-5M cells were transplanted into nude rats, where the infiltration of neutrophils was observed in and around the transplanted tumor tissue. This finding underscores the relevance of KHM-5M as a model for studying the interactions between tumor cells and the immune microenvironment, particularly in relation to neutrophil recruitment and function. The cell line also serves as a valuable tool for investigating the molecular mechanisms underlying cytokine production in cancer and the subsequent modification of pathological features. Through DNA cloning techniques, the chemotactic activities attributed to IL-8 and GM-CSF were confirmed, solidifying the KHM-5M cell line as a significant resource for research into cytokine-driven tumor-immune interactions.
Organism	Human
Tissue	Thyroid
Disease	Thyroid gland anaplastic carcinoma
Metastatic site	Pleural effusion
Synonyms	KHM/5M, KHM5M

Age	65 years
Gender	Male
Morphology	Fibroblast
Growth properties	Adherent

Citation	KHM-5M (Cytion catalog number 305148)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_2975

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	27 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Flask Coating	None
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305148-060524	Certificate of Analysis	23. May. 2025	305148

KHM-5M Cells

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Certificate of Analysis (CoA)