K562 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300224

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The K562 cell line, originating from the bone marrow of a 53-year-old female with chronic myelogenous leukemia, serves as a cornerstone in various research fields such as immunology, tumor immunology, and immune system disorder research. Human K-562 cells are widely used in studies involving immune system interactions, particularly with effector cells like natural killer cells (NK). This is due to their unique characteristics, such as the expression of specific antigens that can be recognized by NK cells. Exploring the interaction between NK cells and cancerous cell lines like K562 offers insights into immune defense mechanisms. NK cells' ability to recognize and respond to K562 cells varies with the presence of specific markers, which fluctuate throughout the K562 cell cycle. K562 cells are characterized by the presence of the Philadelphia chromosome, which results from a translocation between chromosomes 9 and 22, creating the BCR-ABL fusion gene. This fusion gene is not a normal ABL transcript but a mutated form that is constitutively active and leads to uncontrolled cell proliferation. Analyzing ABL transcripts in K562 cells sheds light on leukemia's molecular dynamics and immune evasion strategies. K562 cells are crucial for understanding the cell cycle, particularly for analyzing cell cycle phases and distributions. This analysis is essential for evaluating the impact of ABL gene expression and the associated decrease in ABL fusion transcripts. Furthermore, K562 cells are valuable in assays assessing the cytotoxic effects of FGFR inhibitors and the activity of epigenetic enzymes, highlighting their significance in elucidating cell signaling pathways and the mechanisms of action of various therapeutic agents. The versatility of K562 cells, ranging from their role in enzyme activity assays to their application in immunological studies with natural killer (NK) cells, emphasizes their widespread utility in the scientific realm. This adaptability highlights their significance in bridging the gap between fundamental research and translational medicine, playing a crucial role in advancing the fight against chronic myelogenous leukemia.
Organism	Human
Tissue	Bone marrow
Disease	Chronic myeloid leukemia
Synonyms	K562, K.562, K 562, KO, GM05372, GM05372E

Age	53 years
Gender	Female
Ethnicity	Caucasian
Morphology	Round cells
Cell type	Lymphoblast
Growth properties	Suspension

Citation	K562 (Cytion catalog number 300224)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0004

Antigen expression	CD7 (25%)
Isoenzymes	G6PD, B, AK-1, 1, ES-D, 1, GLO-1, 2, PGM1, 0, PGM3, 1, Me-2, 0
Oncogenes	BCR-ABL1
Tumorigenic	Yes, in nude mice.
Reverse transcriptase	Negative

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Subculturing	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 10⁵ cells/ml and keep the cell concentration within the range of 3 x 10⁵ to 1 x 10⁶ cells/ml for optimal growth.
Seeding density	3 x 10⁵ cells/ml
Fluid renewal	Every 2 days
Post-Thaw Recovery	Please allow cells to recover for roughly 24 to 48 hours after thawing.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300224-290925	Certificate of Analysis	05. Dec. 2025	300224
300224-270524	Certificate of Analysis	30. Mar. 2026	300224
300224-060923	Certificate of Analysis	23. May. 2025	300224

K562 Cells

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Genotype of the human K562 cell line

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Certificate of Analysis (CoA)