HL-60 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300209

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	HL-60 cells, originating from a 36-year-old female with acute promyelocytic leukemia, serve as a vital model in cancer research, particularly in studying hematological malignancies, due to their ability to differentiate into mature white blood cells and mimic innate immune responses, aiding in the understanding of leukemic progression, cellular oncogene expression, and therapeutic target identification. The ability of HL-60 cells to differentiate into mature white blood cells, such as granulocytes and monocytes, through agents like dimethyl sulfoxide (DMSO) or retinoic acid, underscores their significance in studies related to human myeloid cell differentiation and sheds light on the mechanisms underpinning leukemic progression and the efficacy of therapeutic interventions. HL-60 human myeloid leukemia cells are integral to research focusing on apoptosis, cell activation, and the cell cycle, including the regulation of key oncogenes like the c-myc proto-oncogene and tumor necrosis factor (TNF-alpha). Their capability to form extracellular traps, structures involved in trapping and killing pathogens, which mirrors the innate immune response seen in primary neutrophils, makes HL-60 cells a useful model for studying the immune aspects of leukemia and how leukemic cells interact with the immune system. Moreover, the responsiveness of HL-60 cells to signaling pathways such as the MAPK pathway and various kinases is crucial for dissecting the molecular mechanisms driving leukemic cell proliferation and differentiation. This aspect is particularly beneficial for identifying therapeutic targets and developing new treatment strategies for leukemia. HL-60 cells are a critical resource in cancer research, offering insights into hematological malignancies, leukemic progression, and potential therapeutic targets through their unique differentiation capabilities and mimicry of immune responses.
Organism	Human
Tissue	Blood
Disease	Acute promyelocytic leukemia
Applications	Transfection host
Synonyms	HL 60, HL.60, HL60

Age	36 years
Gender	Female
Ethnicity	Caucasian
Morphology	Round cells
Cell type	Lymphoblast
Growth properties	Suspension

Citation	HL-60 (Cytion catalog number 300209)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0002

Receptors expressed	Complement, Fc
Isoenzymes	G6PD, B, PGM1, 1, PGM3, 1, ES-D,1, Me-2, 1, AK-1, 1, GLO-1, 1
Oncogenes	Myc+
Reverse transcriptase	Negative
Products	Tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% heat-inactivated FBS
Subculturing	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 10⁵ cells/ml and keep the cell concentration within the range of 3 x 10⁵ to 1 x 10⁶ cells/ml for optimal growth.
Seeding density	2 x 10⁵ cells/ml
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300209-221223	Certificate of Analysis	23. May. 2025	300209
300209-040325	Certificate of Analysis	23. May. 2025	300209

HL-60 Cells

Summary on the HL-60 cell line

Characteristics

Specifications

Genetics of the promyelocytic leukemia cell line HL-60

Handling

Quality control

Certificate of Analysis (CoA)