HK-2 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305021

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The HK-2 cell line is a well-characterized human proximal tubular epithelial cell line derived from normal adult kidney tissue. These cells exhibit typical epithelial morphology and retain many of the biochemical and functional properties of proximal tubular cells, making them a valuable model for studying renal physiology and pathophysiology. HK-2 cells are known for their ability to perform active transport and exhibit brush border enzyme activities, which are essential for their role in renal reabsorption processes. HK-2 cells express a range of transporters and receptors, including those for glucose, amino acids, and various ions, reflecting their role in renal filtration and reabsorption. They are also responsive to hormonal regulation, such as by parathyroid hormone and aldosterone, which influence their transport activities. Due to these characteristics, HK-2 cells are extensively used in nephrotoxicity studies, drug screening, and research on renal diseases such as acute kidney injury and chronic kidney disease. Moreover, HK-2 cells have been utilized in studies investigating renal cell carcinoma and other kidney-related cancers. They provide a reliable in vitro system for examining cellular responses to toxic agents, oxidative stress, and hypoxia. Researchers also employ HK-2 cells to explore the molecular mechanisms underlying fibrosis and inflammation in the kidney. Overall, the HK-2 cell line is a critical tool in renal research, offering insights into both normal kidney function and disease pathogenesis.
Organism	Human
Tissue	Kidney, cortex, proximal tubule
Synonyms	Hk-2, HK2, Human Kidney-2

Age	Adult
Gender	Male
Ethnicity	European
Morphology	Epithelial
Growth properties	Adherent

Citation	HK-2 (Cytion catalog number 305021)
Biosafety level	HK-2 cells are generally classified as Biosafety Level 1 in Germany (ZKBS). However, due to their immortalization with HPV-16 oncogenes, some institutions may handle them at Biosafety Level 2 as a precaution. Consult local biosafety guidelines for specific handling procedures.
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0302

Receptors expressed	Epidermal growth factor(EGF), expressed
Protein expression	Alkaline Phosphatase, Gamma Glutamyltranspeptidase, Leucine Aminopeptidase, Acid Phosphatase, Cytokeratin, Alpha 3, Beta 1 Integrin, Fibronectin

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305021-280725	Certificate of Analysis	22. Oct. 2025	305021
305021-030624	Certificate of Analysis	23. May. 2025	305021
305021-041022	Certificate of Analysis	23. May. 2025	305021
305021-020224	Certificate of Analysis	23. May. 2025	305021

HK-2 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)