HCC70 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$759.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305464

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The HCC70 cell line is derived from a triple-negative breast cancer (TNBC), a subtype that lacks expression of estrogen, progesterone, and HER2 receptors, making it difficult to treat due to limited targeted therapies. HCC70 cells are notable for their basal-like 1 (BL1) classification within TNBC subtypes, which impacts their response to chemotherapy and treatment strategies. Importantly, HCC70 cells express the G-protein-coupled estrogen receptor GPR30 at significant levels. GPR30 has been associated with rapid signaling responses to estrogens like 17β-estradiol, influencing cell proliferation and other oncogenic pathways. A key genetic characteristic of HCC70 is the presence of a TP53 mutation, specifically the R248Q variant. This mutation is associated with gain-of-function (GOF) phenotypes that contribute to cancer cell survival and aggressive behavior. In studies, R248Q mutation in HCC70 cells has been linked to enhanced cell deformability and altered PARP1 localization, implying potential sensitivity to PARP inhibitors. Research into drug responses in HCC70 and similar TNBC cell lines has highlighted the efficacy of proteasome inhibitors and platinum-based therapies. These treatments have shown promise, with drugs like bortezomib demonstrating cytotoxic effects. The interplay between chemotherapeutic resistance and specific receptor signaling, such as that mediated by GPR30, underscores the complexity of targeting TNBC subtypes like those modeled by HCC70.
Organism	Human
Tissue	Mammary gland
Disease	Breast ductal carcinoma
Synonyms	HCC-70, HCC 70, HCC0070, Hamon Cancer Center 70

Age	49 years
Gender	Female
Ethnicity	African American
Morphology	Epithelial-like
Cell type	Epithelial cell
Growth properties	Adherent

Citation	HCC70 (Cytion catalog number 305464)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1270

Protein expression	Epithelial glycoprotein 2 (EGP2), cytokeratin 19
Oncogenes	Her2/neu-, p53+ (overexpressed)

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305464-300525	Certificate of Analysis	21. Jul. 2025	305464
305464-051124	Certificate of Analysis	23. May. 2025	305464

HCC70 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)