HCC1143 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$759.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305545

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The HCC1143 cell line is derived from a human triple-negative breast cancer (TNBC), specifically lacking estrogen receptor (ER), progesterone receptor (PR), and HER2 expression. This cell line is known for its use in modeling aggressive breast cancer phenotypes and understanding mechanisms underlying treatment resistance. HCC1143 exhibits distinct characteristics, including heterogeneity in cell subpopulations, contributing to its relevance in research focused on phenotypic plasticity and tumor cell state transitions. Studies utilizing HCC1143 have demonstrated that different cell states within the line can transition between luminal, basal, and mesenchymal differentiation states under therapeutic pressures, highlighting its role in studying therapy-induced phenotypic changes and drug resistance mechanisms. HCC1143 cells have been used in various experimental contexts, including investigations of resistance mechanisms to chemotherapy agents like paclitaxel. Single-cell RNA sequencing (scRNA-seq) has revealed subpopulations with differential gene expression profiles linked to treatment resistance. For example, specific subpopulations such as AKR1C3+, IDO1+, and HEY1+ cells have shown increased representation following prolonged paclitaxel treatment, suggesting their role as drug-resistant phenotypes. These subtypes are associated with pathways involving reactive oxygen species (ROS), inflammatory responses, and cell cycle regulation, indicating complex adaptations that facilitate survival under chemotherapeutic stress. Research on HCC1143 has also extended to targeted therapy studies. The application of inhibitors targeting components like ADAM-17 has shown potential in reducing the invasiveness and proliferation of this cell line, supporting its application as a model for testing novel anticancer strategies. These findings underscore HCC1143’s value for exploring both therapeutic responses and the underlying cellular dynamics that drive drug resistance in TNBC.
Organism	Human
Tissue	Breast
Disease	Carcinoma
Synonyms	HCC-1143, Hamon Cancer Center 1144

Age	52 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Cell type	Epithelial cell
Growth properties	Adherent

Citation	HCC1143 (Cytion catalog number 305545)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1245

Protein expression	Epithelial glycoprotein 2 (EGP2), cytokeratin 19
Oncogenes	Her2/neu-, p53+
Mutational profile	Mutation: TP53, p.Arg248Gln (c.743G>A), homozygous

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with TrypLE Express, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	3 to 4 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305545-031125	Certificate of Analysis	05. Dec. 2025	305545

HCC1143 Cells

General information

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Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)