DSL-6B-C2 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 500167

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The DSL-6B/C2 cell line is derived from the DSL-6 transplantable acinar cell carcinoma of the pancreas, specifically established from a tumor model in a male Lewis rat. This model was initiated in 1986 from a primary acinar cell carcinoma that developed after intraperitoneal administration of azaserine, a potent carcinogen. The significance of this cell line stems from its origin in pancreatic cancer research, highlighting its utility in studying the biology and underlying mechanisms of pancreatic acinar cell carcinomas. Initially, upon establishment in culture, DSL-6B/C2 cells exhibited the characteristic production of amylase, a hallmark of pancreatic exocrine function. However, this exocrine enzyme production was transient, ceasing within one to two weeks of culture. This change in phenotypic expression is notable as it suggests an adaptation to the in vitro environment, which may affect the cells' utility in certain types of biological assays. The loss of amylase production might also reflect changes in cell differentiation or the emergence of subpopulations within the cultured cells, which could be critical for researchers focusing on the evolution of tumor cell characteristics in vitro.
Organism	Rat
Tissue	Pancreas
Disease	Carcinoma
Metastatic site	Ductal
Synonyms	DSL-6B/C2, DSL6B/C2

Breed/Subspecies	Lewis
Age	2 years
Gender	Male
Morphology	Epithelial-like
Cell type	Acinar cells
Growth properties	Adherent

Citation	DSL-6B-C2 (Cytion catalog number 500167)
Biosafety level	1
NCBI_TaxID	10116
CellosaurusAccession	CVCL_4167

Tumorigenic	Yes, in Lewis rats the cells produce solid tumors and partially cystic tumors composed with a mixed phenotype of squamous, mucinous and glandular areas
Products	Mucin

Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 x 10⁴ cells/cm² will yield in a confluent layer in about 4 days
Fluid renewal	2 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
500167-412	Certificate of Analysis	18. Aug. 2025	500167
500167-516SF	Certificate of Analysis	23. May. 2025	500167

DSL-6B-C2 Cells

General information

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Regulatory Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)