DMS-79 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$759.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300164

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	DMS-79 is a human lung cancer cell line derived from a small cell lung carcinoma. These cells exhibit a classical neuroendocrine phenotype, which is characteristic of small cell lung cancer. This phenotype is significant because it implies a potential utility in studying neuroendocrine signaling pathways, which are crucial in the development and progression of lung cancer. The DMS-79 cell line has been widely utilized in research to understand the molecular biology of lung cancers, particularly in the context of tumor genesis, cell proliferation, and apoptosis. The cell line is known for its aggressive growth and high tumorigenicity in vivo, making it an excellent model for in vivo studies of tumor behavior and response to therapeutics. DMS-79 cells also serve as a useful tool for pharmacological testing and drug development, offering insights into the cellular responses to various chemotherapeutic agents. Furthermore, these cells have been instrumental in the study of cancer stem cell characteristics and mechanisms of metastasis in small cell lung carcinoma. This extensive usage underscores the importance of DMS-79 in cancer research, particularly in therapies targeting aggressive and hard-to-treat cancers like small cell lung carcinoma.
Organism	Human
Tissue	Lung
Disease	Carcinoma, azaserine induced
Metastatic site	Pleural effusion
Synonyms	DMS 79, DMS79

Age	65 years
Gender	Male
Ethnicity	Caucasian
Growth properties	Aggregates in suspension

Citation	DMS-79 (Cytion catalog number 300164)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1178

Receptors expressed	Epidermal growth factor (EGF)
Antigen expression	Leu 7, My23, Class 1 HLA, Class 2 HLA
Oncogenes	C-myc +, N-myc +, c-raf-1 +, Ha-ras +, Ki-ras +, N-ras +, v-fes -, v-fms -
Tumorigenic	Yes, in nude mice
Products	Adrenocorticotropin (adrenocorticotropic hormone, ACTH), bombesin, calcitonin, corticotropin, beta endorphin, 17 beta estradiol, lipotropin, oxytocin - neurophysin (OT-NP), parathormone, somatostatin-like immunoreactivity (SRIF)

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% heat-inactivated FBS, add 2.5 g/L glucose and 10 mM HEPES
Doubling time	96 hours
Subculturing	Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Subculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate at 37 degree Celsius for 10minutes. Collect the cells following centrifugation, resuspend in fresh cell culture medium and count. Start cultures at 2-4 x 10⁴ cells/ml.
Seeding density	2 to 4 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, allow the cells to recover from the freezing process for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300164-270924	Certificate of Analysis	18. Dec. 2025	300164
300164-913	Certificate of Analysis	23. May. 2025	300164

DMS-79 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)