C-33 A Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305072

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	C-33 A cells originate from the cervical tissue of a 66-year-old Caucasian female diagnosed with uterine cancer. This cell line is characterized by a unique genetic alteration in the TP53 gene, where a point mutation at codon 273 results in an arginine to cysteine substitution, leading to elevated expression of the p53 protein. This mutation plays a critical role in the pathophysiology of the cells, influencing their growth properties and tumorigenic potential. Notably, C-33 A cells are confirmed to be tumorigenic. When introduced into immunodeficient nude mice, these cells have the capability to form undifferentiated carcinomas, which highlights their utility in cancer research, particularly in studies aimed at understanding the mechanisms of tumor initiation and progression in cervical cancer. Furthermore, these cells are negative for both DNA and RNA of human papillomavirus (HPV), distinguishing them from many other cervical cancer cell lines which often carry HPV integrations. This aspect makes C-33 A cells particularly valuable for studying cervical cancer that develops independently of HPV infection, offering insights into alternative pathways of carcinogenesis.
Organism	Human
Tissue	Cervix
Disease	Squamous cell carcinoma of the cervix uteri
Synonyms	C33A, C33a, C33-A, C-33-A, C-33A, C33

Age	66 years
Gender	Female
Morphology	Epithelial
Growth properties	Adherent

Citation	C33A (Cytion catalog number 305072)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1094

Protein expression	Oncogenes: P53＋, Prb＋
Tumorigenic	Yes

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305072-180324	Certificate of Analysis	23. May. 2025	305072

C-33 A Cells

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Certificate of Analysis (CoA)