Bend.3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305265

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The Bend.3 cell line is derived from mouse brain endothelial cells and is widely utilized in neurovascular research. These cells serve as a model for studying the blood-brain barrier (BBB), a critical structure that regulates the passage of substances from the bloodstream into the brain. Bend.3 cells are instrumental in exploring the molecular and cellular mechanisms governing BBB integrity, permeability, and transport functions. Researchers use Bend.3 cells to investigate the pathophysiology of various neurological disorders, such as stroke, Alzheimer's disease, and multiple sclerosis, where BBB dysfunction is a hallmark. Bend.3 cells exhibit endothelial characteristics, including the expression of tight junction proteins such as occludin, claudins, and zonula occludens-1 (ZO-1), which are essential for maintaining the selective permeability of the BBB. They also express markers like CD31 and von Willebrand factor, typical of endothelial cells. Bend.3 cells respond to inflammatory stimuli and oxidative stress, making them suitable for studies on BBB disruption and neuroinflammation. Additionally, this cell line is used to assess the efficacy and safety of pharmacological agents intended to cross the BBB, aiding in the development of treatments for central nervous system disorders. The utility of Bend.3 cells in modeling the neurovascular unit underscores their importance in advancing our understanding of brain endothelial cell biology and the development of neurotherapeutics.
Organism	Mouse
Tissue	Brain, cerebral cortex
Disease	Endothelioma
Synonyms	bEND.3, b.End3, bEnd.3, bEnd3, BEND3, brain-derived Endothelial cells.3

Breed/Subspecies	BALB/c
Age	6 weeks
Gender	Unspecified
Morphology	Endothelial
Cell type	Endothelial cell
Growth properties	Adherent

Citation	Bend.3 (Cytion catalog number 305265)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_0170
GMO Status	GMO-S1: This murine endothelial cell line (bEnd.3) contains a polyomavirus middle T antigen encoded by the NTKmT retroviral vector, driving transformation and enhanced proliferation. The construct is stably present in brain microvascular endothelial cells. This classification applies only within Germany and may differ elsewhere.

Antigen expression	ICAM-1 +, VCAM-1 +, MAdCAM-1 +
Viruses	Transformant: Murine polyomavirus (strain A2) (MPyV) middle T antigen (PyMT)

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305265-260525	Certificate of Analysis	18. Aug. 2025	305265

Bend.3 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)