3T3-Swiss albino Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 400103

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The 3T3-Swiss Albino cell line is a fibroblast cell line derived from the tissues of a Swiss albino mouse embryo. Developed in the 1960s by George Todaro and Howard Green, this line was one of the first to be established for the purpose of long-term cultivation and research of fibroblast cells. The name "3T3" refers to the protocol used for subculturing these cells: "3" days interval and "T3" for the population density at which cells were seeded (3 x 10⁵ cells per 20 cm² flask). 3T3-Swiss Albino cells are commonly used as a model system for studying fibroblast biology, including cellular aging, transformation, and the effects of various pharmaceuticals and toxins on cellular health and replication. They are particularly noted for their robustness and reliability in supporting the replication of various mammalian viruses and for producing viral vaccines. Additionally, these cells are instrumental in cancer research, providing a consistent model for examining the cellular mechanisms of oncogenesis and the interaction of cancer cells with connective tissue environments. Genetically, 3T3-Swiss Albino cells are characterized by a stable karyotype, which facilitates their use in genetic studies. They are highly adaptable to various in vitro conditions, making them extremely valuable for genetic, cytological, and biochemical studies. Their role in the development of biomedical research cannot be overstated, providing crucial insights into cellular processes and potential therapeutic targets in various diseases.
Organism	Mouse
Tissue	Embryonic
Applications	This cells have been used to study cancer development and progression, embryonic development and differentiation, signaling pathways involved in cellular processes such as cell growth and differentiation, and as a substrate for the production of monoclonal antibodies and the expression of recombinant proteins for production and purification.
Synonyms	3T3 Swiss Albino, 3T3, Swiss-3T3, Swiss 3T3, Swiss3T3

Breed/Subspecies	Swiss albino
Age	Embryo
Gender	Male
Morphology	Fibroblast-like
Cell type	Fibroblast
Growth properties	Adherent

Citation	3T3-Swiss Albino (Cytion catalog number 400103)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_0120

Tumorigenic	No
Viruses	Tested and found negative for ectromelia virus (mousepox).
Virus susceptibility	Polyomavirus, SV40
Reverse transcriptase	Negative
Products	T
Ploidy status	Hypertriploid
Karyotype	2n=40

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	18 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	0,5 to 3 x 10⁴ cells/cm²
Fluid renewal	2 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Info: Cells are contact inhibited, subcultivate at a maximum confluence of 80%.

Lot Number	Certificate Type	Date	Catalog Number
400103-030226	Certificate of Analysis	05. Mar. 2026	400103

3T3-Swiss albino Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)