Y3-Ag 1.2.3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€430.00*

Product number: 305207

Description	This cell line is derived from an azaguanine-resistant mutant of S210 myeloma. The cells are resistant to 8-azaguanine but sensitive to HAT. The cells can be used as a rat B cell fusion partner for preparing rat-rat hybridomas. Commercial use or third party distribution has to be permitted by C. Milstein.
Organism	Rat
Tissue	Plaemocytoma, Myeloma
Disease	Rat plasma cell myeloma
Synonyms	Y3.AG.1.2.3, Y3-Ag1.2.3, Y3-Ag1,2,3, Y3Ag1.2.3, Y-3-Ag 1.2.3, 210-RC Y3-Ag 1,2,3, 210RCY3-Ag1.2.3, 210RCY3-Ag123, Y3-Ag123, Y3, Y3M

Protein expression	Immunoglobulin

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Subculturing	Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
Split ratio	1?10^5 to 1?10^6 cells/mL
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305207-020924	Certificate of Analysis	23. May. 2025	305207

General information