SW756 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305588

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	SW756 is a human cervical carcinoma cell line derived from a primary squamous cell carcinoma of the cervix in an adult female. It was included in early comprehensive cell line characterization efforts and has been validated as free from HeLa contamination based on glucose-6-phosphate dehydrogenase (G6PD) isoenzyme typing, which confirmed it to be type B, unlike HeLa cells, which are type A. This ensures its authenticity as a distinct cervical cancer model. SW756 is classified as a squamous cell carcinoma and has been used in various genomic and proteomic profiling studies, including large-scale pharmacogenomic and functional genomics datasets. In proteomic profiling efforts such as the ProCan-DepMapSanger dataset, SW756 contributes to a broader understanding of protein expression regulation, particularly in relation to post-transcriptional modifications and drug response correlations. Such datasets have shown that proteomic data from SW756, like other cell lines, can reveal patterns of lineage-specific expression and regulatory mechanisms that are not always evident at the transcriptomic level, highlighting its utility in integrated multi-omic analyses.
Organism	Human
Tissue	Uterus, cervix
Disease	Squamous cell carcinoma
Synonyms	SW-756, SW 756

Age	46 years
Gender	Female
Ethnicity	Caucasian
Growth properties	Adherent

Citation	SW756 (Cytion catalog number 305588)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1727

Mutational profile	Mutation: p.Gly12Cys, Heterozygous

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	1.6 days
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Lot Number	Certificate Type	Date	Catalog Number
305588-160925	Certificate of Analysis	05. Dec. 2025	305588

SW756 Cells

General information

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Regulatory Data

Biomolecular Data

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Quality control / Genetic profile / HLA

Certificate of Analysis (CoA)