RKO-AS45-1 Cells


General information
Description | The RKO-AS45-1 cell line was generated from the colon carcinoma RKO cell line by transfection with the open reading frame portions of human cDNAs for GADD45 cloned into the expression vector pCMV. |
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Organism | Human |
Tissue | Colon |
Disease | Colon carcinoma |
Synonyms | RKO-AS-45-1, RKOAS451, RKO_AS45 |
Characteristics
Morphology | Epithelial |
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Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | RKO-AS45-1 (Cytion catalog number 305146) |
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Biosafety level | 2 |
Expression / Mutation
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:5 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 8,11
D13S317: 8,11
D16S539: 12.1,13
D5S818: 11,13
D7S820: 8,1
TH01: 6,1
TPOX: 10,11
vWA: 16,24
D3S1358: 15,16,18
D21S11: 28,3
D18S51: 11,12
Penta E: 11,13
Penta D: 10,11.1
D8S1179: 9,13,14
FGA: 20,22
D6S1043: 14.1,20
D2S1338: 16
D12S391: 15,2
D19S433: 14
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