NCI-H2452 Cells
General information
Organism | Human |
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Tissue | Lung |
Disease | Pleural biphasic mesothelioma |
Synonyms | NCI-H2452, H-2452, NCIH2452 |
Characteristics
Age | Adult |
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Gender | Male |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | NCI-H2452 (Cytion catalog number 300391) |
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Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
TH01: 6,9.3
TPOX: 8,11
vWA: 17,18
D3S1358: 17
D21S11: 28,32.2
D18S51: 15
Penta E: 12,15
Penta D: 9
D8S1179: 10
FGA: 23
D6S1043: 11,12
D2S1338: 20
D12S391: 17.3,21
D19S433: 13
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