Human Subcutaneous Pre-Adipocytes

€800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300697

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Material Transfer Agreement

Description	Human Subcutaneous Pre-Adipocytes are primary preadipocytes isolated from human subcutaneous adipose tissue. They can be expanded and differentiated into mature adipocytes, serving as a model for adipogenesis, metabolism, obesity, and diabetes research.
Organism	Human
Tissue	Adipose tissue (subcutaneous)
Disease	Normal
Applications	Adipogenesis and differentiation studies; obesity, metabolism and diabetes research

Morphology	Fibroblast-like (preadipocyte)
Cell type	Preadipocyte
Growth properties	Adherent

Citation	Human Subcutaneous Pre-Adipocytes (Cytion catalog number 300697)
Biosafety level	1
NCBI_TaxID	9606

Culture Medium	Preadipocyte Growth Medium
Supplements	Growth supplements; serum (FBS) as per medium
Dissociation Reagent	Trypsin-EDTA or Accutase
Doubling time	Approximately 48-72 hours
Subculturing	At 70-80% confluence, wash with PBS, detach with Trypsin-EDTA or Accutase, neutralize, centrifuge at 300xg for 3 min, and reseed into fresh medium.
Split ratio	1:2 to 1:3
Seeding density	5 x 10^3 cells/cm2
Fluid renewal	Every 2-3 days
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Lot Number	Certificate Type	Date	Catalog Number
300697-SK0284	Certificate of Analysis	25. Feb. 2026	300697

If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.

For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.

Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.

Required products

Required products

FBS, Fetal Bovine Serum, US-Origin, 50 ml

Sourced from the United States, this sterile-filtered Fetal Bovine Serum (FBS) provides a consistent foundation for cell culture applications. Every batch undergoes quality control screening for sterility, mycoplasma, and bovine viruses. Additionally, each lot is biologically validated to confirm its ability to support the growth of CHO and HEK293 cell lines. The serum is packaged in 50 mL and 500 mL volumes. Batch-specific Certificates of Analysis (CoA) are available upon request.

Product Specifications

Appearance
Clear amber liquid

Storage and shelf life
Store at ≤-15°C.Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended.Once opened store at 4°C and use within 4-6 weeks.

Shipping conditions
Frozen (Dry ice)

Thawing
Overnight at +2°C to +8°C. Swirl gently to homogenize.

€52.00*

Human Subcutaneous Pre-Adipocytes

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)

Material Transfer Agreement