Human Subcutaneous Pre-Adipocytes
General information
| Description | Human Subcutaneous Pre-Adipocytes are primary preadipocytes isolated from human subcutaneous adipose tissue. They can be expanded and differentiated into mature adipocytes, serving as a model for adipogenesis, metabolism, obesity, and diabetes research. |
|---|---|
| Organism | Human |
| Tissue | Adipose tissue (subcutaneous) |
| Disease | Normal |
| Applications | Adipogenesis and differentiation studies; obesity, metabolism and diabetes research |
Characteristics
| Morphology | Fibroblast-like (preadipocyte) |
|---|---|
| Cell type | Preadipocyte |
| Growth properties | Adherent |
Regulatory Data
| Citation | Human Subcutaneous Pre-Adipocytes (Cytion catalog number 300697) |
|---|---|
| Biosafety level | 1 |
| NCBI_TaxID | 9606 |
Biomolecular Data
Handling
| Culture Medium | Preadipocyte Growth Medium |
|---|---|
| Supplements | Growth supplements; serum (FBS) as per medium |
| Dissociation Reagent | Trypsin-EDTA or Accutase |
| Doubling time | Approximately 48-72 hours |
| Subculturing | At 70-80% confluence, wash with PBS, detach with Trypsin-EDTA or Accutase, neutralize, centrifuge at 300xg for 3 min, and reseed into fresh medium. |
| Split ratio | 1:2 to 1:3 |
| Seeding density | 5 x 10^3 cells/cm2 |
| Fluid renewal | Every 2-3 days |
| Freeze medium | As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability. |
| Thawing and Culturing Cells |
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| Incubation Atmosphere | 37°C, 5% CO2, humidified atmosphere. |
| Shipping Conditions | Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage. |
| Storage Conditions | For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen. |
Quality Control & Molecular Analysis
Certificate of Analysis (CoA)
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 300697-SK0284 | Certificate of Analysis | 25. Feb. 2026 | 300697 |
Material Transfer Agreement
If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.
For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.
Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.