HARA-B Cells
General information
Organism | Human |
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Tissue | Lung |
Disease | Lung squamous cell carcinoma |
Metastatic site | Pleural effusion |
Synonyms | HARAB |
Characteristics
Age | 57 years |
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Gender | Male |
Ethnicity | Japanese |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HARA-B (Cytion catalog number 300465) |
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Expression / Mutation
Protein expression | Produces high level of parathyroid hormone-related peptide (PTHrP). |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 13
D13S317: 9
D16S539: 10
D5S818: 12
D7S820: 12
TH01: 7
TPOX: 8,9
vWA: 16,17
D3S1358: 15
D21S11: 30
D18S51: 13
Penta E: 11
Penta D: 9
D8S1179: 10,12
FGA: 20
D6S1043: 11,14
D2S1338: 19,20
D12S391: 18,19
D19S433: 13
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