CHO-BCMA Cells
€1,900.00*
Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 106 cells for adherent lines or 5 × 106 cells for suspension lines (refer to the batch CoA for details).
General information
| Description | Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. CHO-BCMA is a stable recombinant Chinese hamster ovary (CHO) cell line engineered to express full-length human B-cell maturation antigen (BCMA; TNFRSF17/CD269). The cell line was generated using a site-specific landing pad recombination system that enables reproducible genomic integration and stable target expression. CHO-BCMA cells are maintained under biosafety level 1 conditions and are cultured at 37°C in a humidified 5% CO2 atmosphere. The recommended culture medium is CHO Growth Medium A supplemented with G418 selection antibiotic to support stable long-term target expression. BCMA is a transmembrane receptor predominantly expressed on mature B cells and plasma cells, where it regulates cell survival and proliferation through interaction with the ligands BAFF and APRIL. The receptor is highly relevant in multiple myeloma and other B-cell malignancies, making CHO-BCMA cells useful for therapeutic antibody screening, CAR-T and bispecific T-cell engager development, receptor binding studies, and flow cytometry-based assay development. |
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| Organism | Chinese hamster |
| Tissue | Ovary |
| Disease | Chinese hamster ovary, non-neoplastic; genetically engineered for BCMA (CD269/TNFRSF17) surface expression |
| Applications | Antibody screening; BCMA-targeted therapy development; CAR-T cell validation; multiple myeloma research; flow cytometry |
Characteristics
| Morphology | Epithelial-like |
|---|---|
| Cell type | Epithelial cells |
| Growth properties | Adherent/suspension |
Regulatory Data
| Citation | CHO-BCMA (Cytion catalog number 305972) |
|---|---|
| Biosafety level | 1 |
| NCBI_TaxID | 10029 |
| CellosaurusAccession | CVCL_A8V3 |
| GMO Status | GMO-S1: This CHO cell line contains a BCMA expression cassette supporting receptor-function analyses. This classification applies only within Germany and may differ elsewhere. |
Biomolecular Data
| Receptors expressed | BCMA, TNFRSF17/CD269 |
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Handling
| Culture Medium | For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039) |
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| Supplements | For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. |
| Dissociation Reagent | For adherent cultures: Trypsin-EDTA |
| Doubling time | approx. 14-16 hours |
| Subculturing | For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C for 5-10 minutes, or until the cells detach. Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO2, and change the medium every 2-3 days. |
| Split ratio | A ratio of 1:5 to 1:10 is recommended |
| Seeding density | 2 to 4 x 105 cells/mL |
| Fluid renewal | 2 to 3 times per week |
| Incubation Atmosphere | 37°C, 5% CO2, humidified atmosphere. |
Quality Control & Molecular Analysis
Material Transfer Agreement
If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.
For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.
Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.