CFPAC-1 Cells












General information
Description | CFPAC-1 cells, derived from a 26-year-old male with cystic fibrosis and liver metastasis of ductal adenocarcinoma, are a hyperdiploid cell line with notable features for biological research. Their adherence growth property and tumorigenic capability in nude mice make them a practical model for in vitro cancer studies. The cell line's karyotype includes a modal number of 73 chromosomes with several translocations, and importantly, two to three copies of chromosome 7, where the cystic fibrosis gene is located. These cells express cancer-related antigens and genes like CA19-9, carcinoembryonic antigen (CEA), pancreatic oncofetal antigen (POA), adenocarcinoma associated antigen (ACAA), and epithelial keratins, offering insights into cancer biology. In terms of cystic fibrosis pathology, CFPAC-1 cells demonstrate unique ion transport activities. They do not respond to cAMP agonists, adenyl cyclase stimulators, or phosphodiesterase inhibitors for chloride ion flux but show increased chloride efflux in response to calcium ionophores. CFPAC-1 cells carry the common cystic fibrosis mutation - deletion of three nucleotides leading to phenylalanine absence at position 508 in the CFTR gene. Morphologically, they exhibit epithelial features with apical microvilli, tight junctions, and gap junctions, relevant for studying epithelial tissue interactions in both cancer and cystic fibrosis. |
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Organism | Human |
Tissue | Pancreas |
Disease | Cystic fibrosis, Pancreatic ductal adenocarcinoma |
Metastatic site | Liver |
Synonyms | CFPac-1, CF PAC-1, CF-PAC1, CF-Pac1, CF Pac1, CFPAC1, CFPac1, CFPAC |
Characteristics
Age | 26 years |
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Gender | Male |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | CFPAC-1 (Cytion catalog number 305066) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | Carcinoembryonic Antigen(Cea), 9Ng/Ml, Pancreatic Oncofetal Antigen(Poa), 28Ng/Ml, Adenocarcinoma Associated Antigen(Acaa), 5000Ng/Ml, Ca 19-9 Antigen, 12000 Units/Ml, Epithelial Keratins |
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Antigen expression | CA19-9 antigen, 12000 units/mL, epithelial keratins |
Tumorigenic | Yes |
Handling
Culture Medium | IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10
D13S317: 12
D16S539: 9,11
D5S818: 10,11
D7S820: 8,1
TH01: 8
TPOX: 8
vWA: 17
D3S1358: 16
D21S11: 30,31.2
D18S51: 12
Penta E: 10,12
Penta D: 11,13
D8S1179: 11,15
FGA: 21,22
D6S1043: 20
D2S1338: 18,23
D12S391: 17
D19S433: 13,15
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