B-LCL-HROM01 Cells


General information
Description | The cell line was derived from the peripheral blood of a patient with CRC. |
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Organism | Human |
Tissue | Peripheral blood |
Disease | Carcinoma |
Synonyms | TiBc HROM |
Characteristics
Morphology | Round cells |
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Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | B-LCL-HROM01 (Cytion catalog number 302079) |
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Biosafety level | 2 |
Depositor | M. Linnebacher |
Expression / Mutation
Viruses | Transformant: EBV |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% heat-inactivated FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Freeze medium | CM-1 (Cytion catalog number 800100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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HLA alleles |
A*: '01:01:01, '31:01:02
B*: '08:01:01, '14:02:01
C*: '07:01:01, '08:02:01
DRB1*: '03:01:01, '13:03:01
DQA1*: '05:01:01, '05:05:01
DQB1*: '02:01:01, '03:01:01
DPB1*: '01:01:01, '04:02:01
E: '01:01:01
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